BPOZ Antibody

Images

 
Western Blot: BPOZ Antibody [NB100-836] - Staining of Human Heart lysate with antibody at 1 ug/mL (RIPA buffer, 35 ug total protein). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: BPOZ Antibody [NB100-836] - Staining of paraffin embedded Human Cerebellum with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: BPOZ Antibody [NB100-836] - Staining of paraffin embedded Human Heart with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BPOZ Antibody Summary

Immunogen
Peptide with sequence RALEDLLVSIGLDC corresponding to C-Terminus according to NP_115937.1, NP_742024.1, NP_742025.1.
Specificity
This antibody is expected to recognise all reported isoforms (NP_115937.1; NP_742024.1; NP_742025.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ABTB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/mL
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
Control
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-836.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BPOZ Antibody

  • ankyrin repeat and BTB (POZ) domain containing 1
  • ankyrin repeat and BTB/POZ domain-containing protein 1
  • BPOZMGC20585
  • BTB3
  • EF1ABP
  • elongation factor 1A binding protein
  • Elongation factor 1A-binding protein

Background

This protein has an ankyrin repeat region and two BTB/POZ domains, which are thought to be involved in protein-protein interactions. Expression of the gene is activated by the phosphatase and tensin homolog, a tumor suppressor. Alternate splicing results in three transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BPOZ Antibody (NB100-836)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ABTB1
Entrez
Uniprot
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