BPOZ Antibody

Western Blot: BPOZ Antibody [NB100-836] - Staining of Human Heart lysate with antibody at 1 ug/mL (RIPA buffer, 35 ug total protein). Primary incubated for 1 hour. Detected by chemiluminescence. Approx 38 kDa band observed in Human Heart lysates, and approx. 50 kDa in preliminary testing of MCF7 cell lysate (calculated MW of 37.6 kDa according to NP_115937.1 and 54 kDa according to ; NP_742024.1). An additional band was also consistently observed at 65-70 kDa. and was successfully blocked by incubation with the immunizing peptide.

Immunohistochemistry-Paraffin: BPOZ Antibody [NB100-836] - Staining of paraffin embedded Human Cerebellum with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: BPOZ Antibody [NB100-836] - Staining of paraffin embedded Human Heart with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BPOZ Antibody Summary

• Immunogen: Peptide with sequence RALEDLLVSIGLDC corresponding to C-Terminus according to NP_115937.1, NP_742024.1, NP_742025.1.
• Specificity: This antibody is expected to recognise all reported isoforms (NP_115937.1; NP_742024.1; NP_742025.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ABTB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/mL
• Control:

  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BPOZ Antibody

• ankyrin repeat and BTB (POZ) domain containing 1
• ankyrin repeat and BTB/POZ domain-containing protein 1
• BPOZMGC20585
• BTB3
• EF1ABP
• elongation factor 1A binding protein
• Elongation factor 1A-binding protein

Background

This protein has an ankyrin repeat region and two BTB/POZ domains, which are thought to be involved in protein-protein interactions. Expression of the gene is activated by the phosphatase and tensin homolog, a tumor suppressor. Alternate splicing results in three transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BPOZ Antibody (NB100-836)(1)

Publication using NB100-836

• Unoki M, Nakamura Y. Growth-suppressive effects of BPOZ and EGR2, two genes involved in the PTEN signaling pathway. Oncogene 2001-07-01 [PMID: 11494141]

Bioinformatics

• Gene Symbol: ABTB1
• Entrez:
  • 80325(Human)
  • 80283(Mouse)
  • 498013(Rat)
• Uniprot:
  • NP_115937.(Human)