NAC1 Antibody (1243A) [Unconjugated]

Western blot shows lysates of D3 mouse embryonic stem cell line, F9 mouse teratocarcinoma stem cells, and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for NAC1 at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

NAC1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to developing primary sensory neurons in dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysate of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for NAC1 at approximately 69 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western, IHC
• Clone: 1243A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NAC1 Antibody (1243A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant mouse NAC1
  Val457-Gln514
  Accession # Q7TS78
• Specificity: Detects mouse NAC1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Nacc1
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NAC1 Antibody (1243A) [Unconjugated]

• BEND8
• BTB (POZ) domain containing 14B
• BTB/POZ domain-containing protein 14B
• BTBD14B
• BTBD30
• NAC1
• NAC1BEN domain containing 8
• NAC-1FLJ37383
• NACC1
• nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
• nucleus accumbens-associated protein 1
• transcriptional repressor NAC1

Background

NAC1 (Nucleus Accumbens-associated Protein 1) also known as NACC1, is a 527 amino acid (aa) BTB/POZ domain containing protein. Human NAC1 shares 86% and 85% aa sequence identity with mouse and rat NAC1, respectively.  It has been shown to have a role in transcriptional regulation in both pluripotent stem cells and in the brain.  Additionally, NAC1 expression is upregulated in many cancers and this expression has been suggested to correlate with increased aggressiveness in some cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Nacc1
• Uniprot:
  • Q7TS78()