N-Cadherin Antibody [Allophycocyanin]

HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human N-Cadherin APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6426A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.

N-Cadherin knockout HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human N-Cadherin APC-conjugated Monoclonal Antibody (Catalog # FAB6426A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). No staining in the N-Cadherin knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Allophycocyanin

N-Cadherin Antibody [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human N‑Cadherin
  Asp160-Ala724
  Accession # P19022
• Specificity: Detects human, mouse, and rat N‑Cadherin in Western blots. In direct ELISA, less than 1% cross-reactivity with recombinant human (rh) E-Cadherin, rhR-Cadherin, and rhCadherin-13 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CDH2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells
  • Knockout Validated 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for N-Cadherin Antibody [Allophycocyanin]

• ACOGS
• ARVD14
• cadherin 2, type 1, N-cadherin (neuronal)
• Cadherin-2
• calcium-dependent adhesion protein, neuronal
• CD325 antigen
• CD325
• CDH2
• CDHN
• CDw325
• NCAD
• N-cadherin 1
• NCadherin
• N-Cadherin
• Neural cadherin
• neural-cadherin

Background

N-Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130-135 kDa member of the "classical" (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N-Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN-Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15-20 kDa prodomain (aa 26-159) is cleaved by proprotein convertase, and the mature molecule (aa 160-906) is transported to the surface. Mature N-Cadherin contains a 565 aa extracellular region (aa 160-724) that possesses five cadherin domains (aa 160-714), and a 161 aa cytoplasmic tail that undergoes phosphorylation at Tyr785. There is one splice variant that contains a 10 aa substitution for aa 839-906. Over aa 160-724, human N Cadherin shares 98% aa identity with mouse N-Cadherin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for N-Cadherin Antibody (FAB6426A)(1)

Publication using FAB6426A

• Tran M, Choi W, Wszolek M, Navai N, Lee I, Nitti G, Wen S, Flores E, Siefker-Radtke A, Czerniak B, Dinney C, Barton M, McConkey D The p63 protein isoform DeltaNp63alpha inhibits epithelial-mesenchymal transition in human bladder cancer cells: role of MIR-205. J Biol Chem, 2012-12-13;288(5):3275-88. 2012-12-13 [PMID: 23239884] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CDH2
• Uniprot:
  • P19022()