N-Cadherin Antibody (691723) [Unconjugated]

Western blot shows lysates of A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human N-Cadherin Monoclonal Antibody (Catalog # MAB13881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for N-Cadherin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human N‑Cadherin Monoclonal Antibody (Catalog # MAB13881, filled histogram) or isotype control antibody mouse IgM (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).

N-Cadherin was detected in immersion fixed paraffin-embedded sections of Alzheimer's human brain using Mouse Anti-Human N-Cadherin Monoclonal Antibody (Catalog # MAB13881) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 691723
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

N-Cadherin Antibody (691723) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse N-Cadherin
  Asp160-Ala724
  Accession # P19022.4
• Specificity: Detects mouse N-Cadherin in direct ELISAs and Western blots. In direct ELISAs approximately 50% cross-reactivity with recombinant mouse N-Cadherin is observed, and no cross-reactivity with recombinant human (rh) E-Cadherin, rhP-Cadherin, rhVE-Cadherin, rhCadherin-4, -8, -11, -12, or -13 is observed.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for N-Cadherin Antibody (691723) [Unconjugated]

• ACOGS
• ARVD14
• cadherin 2, type 1, N-cadherin (neuronal)
• Cadherin-2
• calcium-dependent adhesion protein, neuronal
• CD325 antigen
• CD325
• CDH2
• CDHN
• CDw325
• NCAD
• N-cadherin 1
• NCadherin
• N-Cadherin
• Neural cadherin
• neural-cadherin

Background

Neuronal Cadherin (N-Cadherin or NCAD), also known as Cadherin-2 (CDH2), is a 130 kDa type I membrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Human N‑Cadherin is synthesized with a 25 amino acid (aa) signal peptide and a 134 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 565 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 161 aa cytoplasmic domain (3). Within the ECD, human N-Cadherin shares 98% aa sequence identity with mouse and rat N-Cadherin. In the nervous system, N-Cadherin mediates adhesion between the opposing faces of developing neuronal synapses and between Schwann cells and neuronal axons (4, 5). It interacts in cis or in trans homophilically and with the GluR2 subunit of neuronal AMPA receptors (1, 6). During synaptic maturation, its expression is lost from inhibitory terminals but maintained at excitatory terminals (5). ADAM10-mediated shedding of the N-Cadherin ECD alters cell-cell adhesion, synaptic development, and AMPA receptor activity (7, 8). N-Cadherin can also be cleaved at multiple additional sites within the intracellular or extracellular domains by Calpain, gamma ‑Secretase, and several MMPs (9 - 13). Cleavage of N‑Cadherin in atherosclerotic plaques contributes alternatively to vascular smooth muscle cell proliferation (MMP-9 and -12) or apoptosis (MMP‑7) (12, 13). Aberrant cell surface expression of the pro and mature forms of N-Cadherin in cancer results in increased tumor progression and invasiveness (14, 15). N-Cadherin also mediates the adhesion between hematopoeitic progenitor cells and mesenchymal stromal cells of the bone marrow (16).

1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
3. Reid, R.A. and J.J. Hemperly (1990) Nucleic Acids Res. 18:5896.
4. Wanner, I.B. and P.M. Wood (2002) J. Neurosci. 22:4066.
5. Benson, D.L. and H. Tanaka (1998) J. Neurosci. 18:6892.
6. Saglietti, L. et al. (2007) Neuron 54:461.
7. Reiss, K. et al. (2005) EMBO J. 24:742.
8. Malinverno, M. et al. (2010) J. Neurosci. 30:16343.
9. Jang, Y.-N. et al. (2009) J. Neurosci. 29:5974.
10. Uemura, K. et al. (2006) Neurosci. Lett. 402:278.
11. Hartland, S.N. et al. (2009) Liver Int. 29:966.
12. Williams, H. et al. (2010) Cardiovasc. Res. 87:137.
13. Dwivedi, A. et al. (2009) Cardiovasc. Res. 81:178.
14. Maret, D. et al. (2010) Neoplasia 12:1066.
15. Tanaka, H. et al. (2010) Nat. Med. 16:1414.
16. Wein, F. et al. (2010) Stem Cell Res. 4:129.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for N-Cadherin Antibody (MAB13881)(7)

Publications using MAB13881

• Park, J;Shim, JK;Lee, M;Kim, D;Yoon, SJ;Moon, JH;Kim, EH;Park, JY;Chang, JH;Kang, SG; Classification of IDH wild-type glioblastoma tumorspheres into low- and high-invasion groups based on their transcriptional program British journal of cancer 8/9/2023 [PMID: 37558923]
• Park, J;Shim, JK;Lee, M;Kim, D;Yoon, SJ;Moon, JH;Kim, EH;Park, JY;Chang, JH;Kang, SG; Classification of IDH wild-type glioblastoma tumorspheres into low- and high-invasion groups based on their transcriptional program British journal of cancer 2023-08-09 [PMID: 37558923]
• Xiaoding Hu, Emilly S. Villodre, Richard Larson, Omar M. Rahal, Xiaoping Wang, Yun Gong et al. Decorin-mediated suppression of tumorigenesis, invasion, and metastasis in inflammatory breast cancer Communications Biology 1/15/2021 [PMID: 33452400]
• Xiaoding Hu, Emilly S. Villodre, Richard Larson, Omar M. Rahal, Xiaoping Wang, Yun Gong et al. Decorin-mediated suppression of tumorigenesis, invasion, and metastasis in inflammatory breast cancer Communications Biology 2021-01-15 [PMID: 33452400] (Western Blot, Human)
• Zhen Zhang, Jing Wang, Karthik Balaji Shanmugasundaram, Belinda Yeo, Andreas Möller, Alain Wuethrich et al. Tracking Drug‐Induced Epithelial–Mesenchymal Transition in Breast Cancer by a Microfluidic Surface‐Enhanced Raman Spectroscopy Immunoassay Small 4/1/2020 [PMID: 32141228]
• E Cherubini, S Mariotta, D Scozzi, R Mancini, G Osman, M D'Ascanio, P Bruno, G Cardillo, A Ricci BDNF/TrkB axis activation promotes epithelial-mesenchymal transition in idiopathic pulmonary fibrosis J Transl Med, 2017-09-22;15(1):196. 2017-09-22 [PMID: 28938915] (Western Blot, Human)
• Yuan Y, Chen H, Ma G, Cao X, Liu Z Reelin is involved in transforming growth factor-β1-induced cell migration in esophageal carcinoma cells. PLoS ONE, 2012-02-29;7(2):e31802. 2012-02-29 [PMID: 22393371] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CDH2
• Uniprot:
  • P19022.4()