N-Cadherin Antibody (691723) [Alexa Fluor® 750]

• Applications: Flow
• Clone: 691723
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

N-Cadherin Antibody (691723) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse N-Cadherin
  Asp160-Ala724
  Accession # P19022.4
• Specificity: Detects mouse N-Cadherin in direct ELISAs and Western blots. In direct ELISAs approximately 50% cross-reactivity with recombinant mouse N-Cadherin is observed, and no cross-reactivity with recombinant human (rh) E-Cadherin, rhP-Cadherin, rhVE-Cadherin, rhCadherin-4, -8, -11, -12, or -13 is observed.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Neuronal Cadherin (N-Cadherin or NCAD), also known as Cadherin-2 (CDH2), is a 130 kDa type I membrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Human N‑Cadherin is synthesized with a 25 amino acid (aa) signal peptide and a 134 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 565 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 161 aa cytoplasmic domain (3). Within the ECD, human N-Cadherin shares 98% aa sequence identity with mouse and rat N-Cadherin. In the nervous system, N-Cadherin mediates adhesion between the opposing faces of developing neuronal synapses and between Schwann cells and neuronal axons (4, 5). It interacts in cis or in trans homophilically and with the GluR2 subunit of neuronal AMPA receptors (1, 6). During synaptic maturation, its expression is lost from inhibitory terminals but maintained at excitatory terminals (5). ADAM10-mediated shedding of the N-Cadherin ECD alters cell-cell adhesion, synaptic development, and AMPA receptor activity (7, 8). N-Cadherin can also be cleaved at multiple additional sites within the intracellular or extracellular domains by Calpain, gamma ‑Secretase, and several MMPs (9 - 13). Cleavage of N‑Cadherin in atherosclerotic plaques contributes alternatively to vascular smooth muscle cell proliferation (MMP-9 and -12) or apoptosis (MMP‑7) (12, 13). Aberrant cell surface expression of the pro and mature forms of N-Cadherin in cancer results in increased tumor progression and invasiveness (14, 15). N-Cadherin also mediates the adhesion between hematopoeitic progenitor cells and mesenchymal stromal cells of the bone marrow (16).

1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
3. Reid, R.A. and J.J. Hemperly (1990) Nucleic Acids Res. 18:5896.
4. Wanner, I.B. and P.M. Wood (2002) J. Neurosci. 22:4066.
5. Benson, D.L. and H. Tanaka (1998) J. Neurosci. 18:6892.
6. Saglietti, L. et al. (2007) Neuron 54:461.
7. Reiss, K. et al. (2005) EMBO J. 24:742.
8. Malinverno, M. et al. (2010) J. Neurosci. 30:16343.
9. Jang, Y.-N. et al. (2009) J. Neurosci. 29:5974.
10. Uemura, K. et al. (2006) Neurosci. Lett. 402:278.
11. Hartland, S.N. et al. (2009) Liver Int. 29:966.
12. Williams, H. et al. (2010) Cardiovasc. Res. 87:137.
13. Dwivedi, A. et al. (2009) Cardiovasc. Res. 81:178.
14. Maret, D. et al. (2010) Neoplasia 12:1066.
15. Tanaka, H. et al. (2010) Nat. Med. 16:1414.
16. Wein, F. et al. (2010) Stem Cell Res. 4:129.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH2