Myeloperoxidase/MPO Antibody (S07-2C7)

Western Blot: Myeloperoxidase/MPO Antibody (S07-2C7) [NBP3-19707] - Western blot detection of Myeloperoxidase/MPO in 3T3 cell lysates using NBP3-19707 (1:1000 diluted). Predicted band size: 84kDa. Observed band size: 80-90kDa.

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (S07-2C7) [NBP3-19707] - Immunohistochemistry of Myeloperoxidase/MPO in paraffin-embedded Human colon cancer tissue using NBP3-19707 at dilution 1/50

• Reactivity: Hu
• Applications: WB, IHC
• Clone: S07-2C7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Myeloperoxidase/MPO Antibody (S07-2C7) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Myeloperoxidase/MPO (Uniprot # P05164)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MPO
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/50-1/200
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000-1/5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Myeloperoxidase/MPO Antibody (S07-2C7)

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MPO