Myeloperoxidase/MPO Antibody (CL14976) - BSA Free

Analysis in human bone marrow and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.

Western blot analysis in human cell line NB4.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human kidney shows no positivity in cells in tubules and glomeruli as expected.

Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.

Staining of human prostate shows no positivity in glandular cells as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL14976
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: .6 mg/ml

Myeloperoxidase/MPO Antibody (CL14976) - BSA Free Summary

• Description: Novus Biologicals Mouse Myeloperoxidase/MPO Antibody (CL14976) - BSA Free (NBP3-26845) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from P05164, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (90%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MPO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: .6 mg/ml
• Purity: Protein A purified

Alternate Names for Myeloperoxidase/MPO Antibody (CL14976) - BSA Free

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MPO