Myeloperoxidase/MPO Antibody (8F4)

Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining in blue. IHC-Fr image submitted by a verified customer review.

• Reactivity: Mu, Rt
• Applications: ELISA, Flow, IHC
• Clone: 8F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Myeloperoxidase/MPO Antibody (8F4) Summary

• Description: Positive Control: Neutrophils isolated from digested infarcts Negative control: Lymphocytes isolated from digested infarcts
• Immunogen: Purified mouse MPO from WEHI-3 cells
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MPO
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunoassay
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin
• Application Notes: Use in IHC-P reported in scientific literature (PMID: 26582929).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Myeloperoxidase/MPO Antibody (8F4)

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO). MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa. MPO is stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms. Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an diagnostic marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myeloperoxidase/MPO Antibody (NBP1-51148)(10)

Publications using NBP1-51148

• Peng W, Qi H, Zhu W et al. Lianhua Qingke ameliorates lipopolysaccharide-induced lung injury by inhibiting neutrophil extracellular traps formation and pyroptosis Pulmonary circulation 2023-10-01 [PMID: 37808899] (IHC-P, Mouse)
• de Oliveira AK, Pramoonjago P, Rucavado A et al. Mapping the Immune Cell Microenvironment with Spatial Profiling in Muscle Tissue Injected with the Venom of Daboia russelii Toxins 2023-03-10 [PMID: 36977099] (Immunohistochemistry-Paraffin, Mouse)
• Zhou X, Zhang C, Wu X et al. Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction Nature communications 2022-11-05 [PMID: 36335128] (IHC-P, Rat)
  
  Details:
  Dilution used in IHC-P 1:50
• Chiara Pavan, Anna L R Xavier, Marta Ramos, Jane Fisher, Marios Kritsilis, Adam Linder, Peter Bentzer, Maiken Nedergaard, Iben Lundgaard DNase Treatment Prevents Cerebrospinal Fluid Block in Early Experimental Pneumococcal Meningitis. Annals of neurology 2021-12-13 [PMID: 34397111]
• Kanzaki G, Nagasaka S, Higo S et al. Impact of anti-glomerular basement membrane antibodies and glomerular neutrophil activation on glomerulonephritis in experimental myeloperoxidase-antineutrophil cytoplasmic antibody vasculitis Nephrol Dial Transplant [PMID: 26582929] (IHC-P, Rat)
  
  Details:
  Used the FITC form of this antibody.
• Nahrendorf, M et al. An activatable MR imaging agent reports myeloperoxidase activity in healing infarcts and detects the anti-inflammatory effects of atorvastatin on ischemia-reperfusion injury non-invasively. Circulation , 117: 1153. 2008-01-01 [PMID: 18268141]
• Matthijsen, M et al. Myeloperoxidase is critically involved in the induction of organ damage after renal ischemia reperfusion. Am J Pathol, 171: 1743. 2007-01-01 [PMID: 18055546]
• Leeuwen van, M et al. Accumulation of myeloperoxidase-positive neutrophils in atherosclerotic lesions in LDLR -/- mice. Arterioscler Thromb Vasc Biol , 28: 84. 2008-01-01 [PMID: 17991873]
• Wang, D et al. C/EBPalpha directs monocytic commitment of primary myeloid progenitors. Blood , 108:1223. 2006-01-01 [PMID: 16645168]
• Huugen, D et al. Aggravation of anti-myeloperoxidase antibody-induced glomerulonephritis by bacterial lipopolysaccharide: role of tumor necrosis factor-alpha. Am J Pathol , 167: 47. 2005-01-01 [PMID: 15972951]

Reviews for Myeloperoxidase/MPO Antibody (NBP1-51148) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Rat.

reviewed by: Vanessa Megaro IHC-Fr Rat 05/07/2021

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: brain and spinal cord
• Species: Rat
• Lot: 23452M1217-A

reviewed by: Verified Customer IHC-Fr Rat 11/19/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Frozen Rat Liver Tissue sections
• Species: Rat
• Lot: 23452M1217-A

Comments

• Comments: 8um section fixed in formalin
  MPO antibody diluted 1:50
  Multiplexed with CD68 antibody

Bioinformatics

• Gene Symbol: MPO