Myeloperoxidase/MPO Antibody (8F4)

Images

 
Immunohistochemistry-Frozen: Myeloperoxidase/MPO Antibody (8F4) [NBP1-51148] - Frozen 8 um rat liver tissue section fixed in formalin. MPO antibody at 1:50 (green). Multiplexed with a CD68 antibody (red). DAPI staining ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications Flow, IA, IHC, IHC-Fr, IHC-P
Clone
8F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Myeloperoxidase/MPO Antibody (8F4) Summary

Immunogen
Purified mouse MPO from WEHI-3 cells
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MPO
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
Application Notes
Use in IHC-P reported in scientific literature (PMID 26582929).
Reviewed Applications
Read 1 Review rated 4
using
NBP1-51148 in the following applications:

Publications
Read Publications using
NBP1-51148 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Notes

Positive Control: Neutrophils isolated from digested infarcts Negative control: Lymphocytes isolated from digested infarcts

Alternate Names for Myeloperoxidase/MPO Antibody (8F4)

  • EC 1.11.1
  • EC 1.11.1.7
  • Lactoperoxidase
  • MPO
  • Myeloperoxidase

Background

The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO). MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa. MPO is stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms. Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an diagnostic marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myeloperoxidase/MPO Antibody (NBP1-51148)(6)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Review for Myeloperoxidase/MPO Antibody (NBP1-51148) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-51148:
Filter by Applications
IHC-Fr
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Myeloperoxidase/MPO NBP1-51148
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reviewed by:
Anonymous
IHC-Fr Rat 11/19/2019
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen Rat Liver Tissue sections
SpeciesRat
Lot23452M1217-A

Comments

Comments8um section fixed in formalin
MPO antibody diluted 1:50
Multiplexed with CD68 antibody

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Recent Reviews

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4
1
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1
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Anonymous
11/19/2019
Application: IHC-Fr
Species: Rat

Bioinformatics

Gene Symbol MPO