Musashi-1 Antibody

Images

 
Western Blot: Musashi-1 Antibody [NBP1-32812] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody [NBP1-32812] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MSI1 protein stained by MSI1 antibody [N3C3] diluted at 1:500. Red: alpha ...read more
Immunohistochemistry-Frozen: Musashi-1 Antibody [NBP1-32812] - Frozen sectioned adult mouse retina. Green: Musashi 1 protein stained by Musashi 1 antibody [N3C3] diluted at 1:250. Red: Protein kinase C alpha staining. ...read more
Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody [NBP1-32812] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Frozen: Musashi-1 Antibody [NBP1-32812] - MSI1 antibody [N3C3] detects MSI1 protein on adult mouse brain by immunohistochemical analysis. Sample: Frozen section of adult mouse brain. MSI1 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.69 mg/ml

Order Details

Musashi-1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Musashi 1. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Marker
Neuronal Stem Cell Marker
Predicted Species
Rat (100%), Porcine (98%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MSI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.69 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Musashi-1 Antibody

  • MSI1
  • Musashi (Drosophila) homolog 1
  • musashi homolog 1 (Drosophila)
  • Musashi1
  • Musashi-1
  • RNA-binding protein Musashi homolog 1

Background

Musashi1, a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, expression of Musashi1 has been detected in human tumor tissues such as gliomas and melanomas, suggesting its involvement in oncogenic development. Results suggest that Musashi1 expression may be an important factor in the development of several types of carcinoma such as human hepatoma, and may be a useful molecular marker for tumor detection. (1). Recent findings demonstrate that translational regulation contributes to cell-fate specification. The evolutionarily conserved, neural RNA-binding protein Musashi1 controls neural cell fate. The protein functions in maintenance of the stem-cell state, differentiation, and tumorigenesis by repressing translation of particular mRNAs. In mammals it might play an important role in activating Notch signaling by repressing translation of the Notch inhibitor m-Numb (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MSI1
Entrez