Musashi-2 Antibody

Western Blot: Musashi 2 Antibody [NBP2-19443] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: Musashi-2 Antibody [NBP2-19443] - Rat brain. Musashi 2 stained by Musashi 2 antibody [N1C1] diluted at 1:500.Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min.

Western Blot: Musashi 2 Antibody [NBP2-19443] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Frozen: Musashi-2 Antibody [NBP2-19443] - Frozen sectioned adult mouse retina. Green: Musashi 2 protein stained by Musashi 2 antibody [N1C1] diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: Musashi-2 Antibody [NBP2-19443] - Rat fore brain. Musashi 2 antibody [N1C1] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Musashi-2 Antibody [NBP2-19443] - Mouse hind brain. Musashi 2 antibody [N1C1] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Bv, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Musashi-2 Antibody Summary

• Description: Novus Biologicals Rabbit Musashi-2 Antibody (NBP2-19443) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-Musashi-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Musashi-2. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Bovine (100%), Xenopus (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MSI2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
• Application Notes: ICC/IF, IP-Assay dependent
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (97%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Musashi-2 Antibody

• FLJ36569
• MGC3245
• MSI2
• MSI2H
• musashi homolog 2 (Drosophila)
• Musashi2
• Musashi-2
• RNA-binding protein Musashi homolog 2

Background

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Musashi-2 Antibody (NBP2-19443)(1)

Publication using NBP2-19443

• Zhu Y, Zhou B, Hu X et al. LncRNA LINC00942 promotes chemoresistance in gastric cancer by suppressing MSI2 degradation to enhance c-Myc mRNA stability Clinical and translational medicine 2022-01-01 [PMID: 35073459] (Human)

Bioinformatics

• Gene Symbol: MSI2
• Uniprot:
  • Q96DH6()