MSX2 Antibody (786607)

MSX2 was detected in immersion fixed SK-BR-3 human breast cancer cell line using Mouse Anti-Human MSX2 Monoclonal Antibody (Catalog # MAB7917) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 786607
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MSX2 Antibody (786607) Summary

• Immunogen: E. coli-derived recombinant human MSX2
  Ala2-Thr132
  Accession # P35548
• Specificity: Detects human MSX2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MSX1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSX2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSX2 Antibody (786607)

• CRS2
• CRS2parietal foramina 1
• FPP
• Homeobox protein Hox-8
• homeobox protein MSX-2
• Hox8
• HOX8msh homeobox homolog 2 (Drosophila)
• msh (Drosophila) homeo box homolog 2
• msh homeo box 2
• msh homeobox 2
• msh homeobox homolog 2
• MSH
• MSX2
• PFM
• PFM1

Background

MSX2, also known as HOX-8, is an approximately 35 kDa homeobox transcription factor that is a downstream target of canonical Wnt signaling. MSX2 plays an important role in the development of mineralized tissues including bone, cartilage, and teeth. It is upregulated in atherosclerotic plaques and promotes the calcification of vascular smooth muscle cells during inflammation. MSX2 suppresses the adipocytic differentiation of multipotent mesenchymal cells. MSX2 acts in mammary gland development and the initiation of female fetal germ cell meiosis. It promotes tumorigenesis of breast and pancreatic carcinomas but inhibits the survival and invasiveness of melanoma cells. Human MSX2 contains one homeodomain (aa 143-201). Within aa 1-132, human MSX2 shares 87% aa sequence identity with mouse and rat MSX2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSX2
• Uniprot:
  • P35548(Human)