MSX1 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse MSX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MSX1 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MSX1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human/Mouse MSX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5045) at 0.3, 1.0 and 3.0 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MSX1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MSX1
  Met1-Thr165
  Accession # P28360
• Specificity: Detects human and mouse MSX1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MSX1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSX1 Antibody [Unconjugated]

• Homeobox protein Hox-7
• homeobox protein MSX-1
• HOX7
• HOX7homeobox 7
• HYD1
• HYD1msh homeobox homolog 1 (Drosophila)
• msh (Drosophila) homeo box homolog 1 (formerly homeo box 7)
• msh homeo box 1
• msh homeobox 1
• Msh homeobox 1-like protein
• msh homeobox homolog 1
• MSX1
• OFC5
• STHAG1

Background

MSX1 (Msh homeobox homology 1) is a member of the muscle segment homoebox gene family. MSX1 is involved in limb-pattern formation, craniofacial development, odontogenesis, and tumor growth inhibition. MSX1 functions as a transcriptional repressor. MSX1 has been shown to interact with the linker histone, H1B, and repress transcription of the MyoD promoter. Chromosomal abnormalities involving MSX1 have been associated with the Wolf-Hirschhorn syndrome characterized by heart defects and mental retardation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MSX1 Antibody (AF5045)(6)

Publications using AF5045

• W Chiu, A Li, T Wang, W Li, X Zhang Generation of a MSX1 knockout human embryonic stem cell line using CRISPR/Cas9 technology Stem Cell Research, 2022-02-26;60(0):102729. 2022-02-26 [PMID: 35247841] (ICC, Human)
• X Hu, C Lin, N Ruan, Z Huang, Y Zhang, X Hu Operation of the Atypical Canonical Bone Morphogenetic Protein Signaling Pathway During Early Human Odontogenesis Frontiers in Physiology, 2022-02-08;13(0):823275. 2022-02-08 [PMID: 35211032] (ICC, IHC, Western Blot, Human)
• S Heino, S Fang, M Lähde, J Högström, S Nassiri, A Campbell, D Flanagan, A Raven, M Hodder, N Nasreddin, HH Xue, M Delorenzi, S Leedham, TV Petrova, O Sansom, K Alitalo Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas Science Advances, 2021-11-17;7(47):eabj0512. 2021-11-17 [PMID: 34788095] (IHC, Mouse)
• L Pellegrini, A Albecka, DL Mallery, MJ Kellner, D Paul, AP Carter, LC James, MA Lancaster SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids Cell Stem Cell, 2020-10-13;0(0):. 2020-10-13 [PMID: 33113348] (IHC, Human)
• M Horazna, L Janeckova, J Svec, O Babosova, D Hrckulak, M Vojtechova, K Galuskova, E Sloncova, M Kolar, H Strnad, V Korinek Msx1 loss suppresses formation of the ectopic crypts developed in the Apc-deficient small intestinal epithelium Sci Rep, 2019-02-07;9(1):1629. 2019-02-07 [PMID: 30733598] (IHC-P, Western Blot, Human, Mouse)
• A Sancho, I Vandersmis, S Craps, A Luttun, J Groll A new strategy to measure intercellular adhesion forces in mature cell-cell contacts Sci Rep, 2017-04-10;7(0):46152. 2017-04-10 [PMID: 28393890] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: MSX1
• Uniprot:
  • P28360()