MSX1 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse MSX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5045) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MSX1 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MSX1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human/Mouse MSX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5045) at 0.3, 1.0 and 3.0 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Regulation of MSX1 expression is mediated by pSMAD1/5/8 in the human cap-stage tooth germs. (A–C) Immunostaining shows that MSX1 expression is abundant in dimethyl sulfoxide (DMSO)-treated human cap-stage tooth germs, dramatically reduced in dorsomorphin treated human cap-stage tooth germ, and unaltered in U0126 + SB203580 + SP600125 treated human cap-stage tooth germs. (D) A Western blot assay confirms the dramatically reduced expression of MSX1 in dorsomorphin treated human cap-stage tooth germs. Actin was used as the internal control. (E) Quantitative analysis of the Western blot assay. de, the dental epithelium; dm, the dental mesenchyme. Error bars represent SD. ***p < 0.001. Bar = 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35211032), licensed under a CC-BY license. Not internally tested by R&D Systems.

Msx1 expression in E12.5 Wls-knockout mutant and control cerebella. In control cerebellum (A,B), Msx1 is expressed in the RL. Expression of Msx1 in the RL is persisted in the Wls knockout cerebellum (C,D) and similar to Msx1 expression in the control RL. RL, rhombic lip. Scale bars, 100 μm. Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/articles/10.3389/fnmol.2024.1356544/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Msx1 and Msx3 expression in E12.5 Atoh1-null and wildtype cerebella. (A,B) The FISH double-labeling of Msx1 and Msx3 in the E12.5 Atoh1-null (a,a’) and wildtype cerebella (b,b’). (a’ and b’) illustrate that the sections from mutant and wildtype are collected at the same medio-lateral position. (C–F) Expression of Msx1 and Msx3 shifts and now sharing a boundary in the Atoh1-null cerebellum as denoted by the red arrow in (C). Panels (A,C–F) indicate Msx1 and Msx3 expression persistence in the Atoh1-null cerebellum. The shift of Msx1 and Msx3 expression, however, does not result in cells that co-express Msx1 and Msx3 (A,C–E). (G–I) A very different picture is shown in the wildtype cerebellum where there is a notable gap between cells that express Msx3 and Msx1 as noted by white arrow in (G). (F,J) DAPI (blue) used as a counterstain. RL, Rhombic Lip; VZ, ventricular zone. All scale bars, 100 μm. Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/articles/10.3389/fnmol.2024.1356544/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Temporal and spatial expression of Msx genes in the developing cerebellum. (A–C) Graphs show the dynamic nature of Msx expression in the cerebellum across 12 developmental time-points as observed from the RIKEN FANTOM5 transcriptome time-course data. (D–I) Sagittal sections of the RL with the right-side of panels denoting posterior and the bottom-side denoting ventral, with RNA in situ hybridization showing Msx genes expressed in the progenitor zones in (D–F) E11.5 and (G–I) E12.5. Msx1 expression is limited to the RL (white arrows in D,G) whereas Msx3 is limited to the VZ (black arrows in E,H). Msx2 expression is detected in the neuroepithelium but the boundary of the expression is not clear (F,I). See supplementary Figure S2 for negative control staining. RL, Rhombic Lip; VZ, Ventricular Zone. Scale bar, 100 μm. Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/articles/10.3389/fnmol.2024.1356544/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MSX1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MSX1
  Met1-Thr165
  Accession # P28360
• Specificity: Detects human and mouse MSX1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MSX1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSX1 Antibody [Unconjugated]

• Homeobox protein Hox-7
• homeobox protein MSX-1
• HOX7
• HOX7homeobox 7
• HYD1
• HYD1msh homeobox homolog 1 (Drosophila)
• msh (Drosophila) homeo box homolog 1 (formerly homeo box 7)
• msh homeo box 1
• msh homeobox 1
• Msh homeobox 1-like protein
• msh homeobox homolog 1
• MSX1
• OFC5
• STHAG1

Background

MSX1 (Msh homeobox homology 1) is a member of the muscle segment homoebox gene family. MSX1 is involved in limb-pattern formation, craniofacial development, odontogenesis, and tumor growth inhibition. MSX1 functions as a transcriptional repressor. MSX1 has been shown to interact with the linker histone, H1B, and repress transcription of the MyoD promoter. Chromosomal abnormalities involving MSX1 have been associated with the Wolf-Hirschhorn syndrome characterized by heart defects and mental retardation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MSX1 Antibody (AF5045)(12)

Publications using AF5045

• Rizzoti, K;Chakravarty, P;Sheridan, D;Lovell-Badge, R; SOX9-positive pituitary stem cells differ according to their position in the gland and maintenance of their progeny depends on context Science advances 2023-10-06 [PMID: 37792947] (IHC, Mouse)
• W Chiu, A Li, T Wang, W Li, X Zhang Generation of a MSX1 knockout human embryonic stem cell line using CRISPR/Cas9 technology Stem Cell Research, 2022-02-26;60(0):102729. 2022-02-26 [PMID: 35247841] (ICC, Human)
• X Hu, C Lin, N Ruan, Z Huang, Y Zhang, X Hu Operation of the Atypical Canonical Bone Morphogenetic Protein Signaling Pathway During Early Human Odontogenesis Frontiers in Physiology, 2022-02-08;13(0):823275. 2022-02-08 [PMID: 35211032] (ICC, IHC, Western Blot, Human)
• S Heino, S Fang, M Lähde, J Högström, S Nassiri, A Campbell, D Flanagan, A Raven, M Hodder, N Nasreddin, HH Xue, M Delorenzi, S Leedham, TV Petrova, O Sansom, K Alitalo Lef1 restricts ectopic crypt formation and tumor cell growth in intestinal adenomas Science Advances, 2021-11-17;7(47):eabj0512. 2021-11-17 [PMID: 34788095] (IHC, Mouse)
• L Pellegrini, A Albecka, DL Mallery, MJ Kellner, D Paul, AP Carter, LC James, MA Lancaster SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids Cell Stem Cell, 2020-10-13;0(0):. 2020-10-13 [PMID: 33113348] (IHC, Human)
• M Horazna, L Janeckova, J Svec, O Babosova, D Hrckulak, M Vojtechova, K Galuskova, E Sloncova, M Kolar, H Strnad, V Korinek Msx1 loss suppresses formation of the ectopic crypts developed in the Apc-deficient small intestinal epithelium Sci Rep, 2019-02-07;9(1):1629. 2019-02-07 [PMID: 30733598] (IHC-P, Western Blot, Human, Mouse)
• Yixuan Chen, Zhengsen Wang, YiPing Chen, Yanding Zhang Conditional deletion of Bmp2 in cranial neural crest cells recapitulates Pierre Robin sequence in mice Cell and Tissue Research 5/1/2019 [PMID: 30413887]
• Moon K H, Kim H T et al. Differential Expression of NF2 in Neuroepithelial Compartments Is Necessary for Mammalian Eye Development. Dev Cell 8/1/2018 [PMID: 29249622]
• A Sancho, I Vandersmis, S Craps, A Luttun, J Groll A new strategy to measure intercellular adhesion forces in mature cell-cell contacts Sci Rep, 2017-04-10;7(0):46152. 2017-04-10 [PMID: 28393890] (ICC, Western Blot, Human)
• Ine Vandersmissen, Sander Craps, Maarten Depypere, Giulia Coppiello, Nick van Gastel, Frederik Maes et al. Endothelial Msx1 transduces hemodynamic changes into an arteriogenic remodeling response Journal of Cell Biology 9/28/2015 [PMID: 26391659]
• Ine Vandersmissen, Sander Craps, Maarten Depypere, Giulia Coppiello, Nick van Gastel, Frederik Maes et al. Endothelial Msx1 transduces hemodynamic changes into an arteriogenic remodeling response Journal of Cell Biology 2015-09-28 [PMID: 26391659] (Immunohistochemistry, Western Blot, Human, Mouse)
• Aranguren X, Agirre X, Beerens M, Coppiello G, Uriz M, Vandersmissen I, Benkheil M, Panadero J, Aguado N, Pascual-Montano A, Segura V, Prosper F, Luttun A Unraveling a novel transcription factor code determining the human arterial-specific endothelial cell signature. Blood, 2013-10-09;122(24):3982-92. 10/9/2013 [PMID: 24108462]

Bioinformatics

• Gene Symbol: MSX1
• Uniprot:
  • P28360()