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Mouse/Rat Leptin Quantikine ELISA Kit

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

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Catalog# & Conjugate Size Price
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Mouse/Rat Leptin Quantikine ELISA Kit Summary

Background
The Quantikine® Mouse/Rat Leptin Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse or rat Leptin in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse Leptin accurately. R...esults obtained for naturally occurring mouse or rat Leptin showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse or rat Leptin.
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Specificity
Natural and recombinant mouse and rat Leptin.
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using MOB00B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat Leptin Quantikine ELISA Kit

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor
  • OBOBS

Background

Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.

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Publications for Leptin/OB (MOB00B)(205)

We have publications tested in 4 confirmed species: Mouse, Rat, Hamster, Transgenic Mouse.


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Mouse
(140)
Rat
(59)
Hamster
(1)
Transgenic Mouse
(6)
All Species
Showing Publications 1 - 10 of 205. Show All 205 Publications.
Publications using MOB00B Applications Species
Hamby, M;Barr, E;Lirette, S;Drummond, HA; Loss of ?ENaC Prevents Hepatic Steatosis but Promotes Abdominal Fat Deposition Associated with a High-Fat Diet Biology 2025-11-06 [PMID: 41300348] (Mouse) Mouse
Hernández-Arciga, U;Kim, JK;Fisher, JL;Tyshkovskiy, A;Moldakozhayev, A;Hall, C;Ghosh, S;Govindaraj, Y;Sipula, IJ;Kastroll, J;Cooke, D;Luo, J;Alder, JK;Sukoff Rizzo, SJ;Ables, GP;Choi, E;Gladyshev, VN;Jurczak, MJ;Tatar, M;Parkhitko, AA; Mutation in IR or IGF1R produces features of long-lived mice while maintaining metabolic health JCI insight 2025-11-11 [PMID: 41217825] (Transgenic Mouse) Transgenic Mouse
Chen, X;Fang, X;Zhou, H;Meng, J;He, Y;Straub, LG;Lemoff, A;Crewe, C;Zhao, S;Xu, Y;Zhu, Y; Regulation of food intake by Connexin43 via adipocyte-sensory neuron electrical synapses Molecular metabolism 2025-09-05 [PMID: 40915562] (Mouse) Mouse
Zhan-Dai, S;Grases-Pintó, B;García-Vara, A;Ferrer, R;Martín-Venegas, R;Lamuela-Raventós, RM;Castell, M;Pérez-Cano, FJ;Vallverdú-Queralt, A;Rodríguez-Lagunas, MJ; Maternal Extra Virgin Olive Oil Supplementation Enhances Offspring Immune Function: A Preclinical Study International journal of molecular sciences 2025-08-18 [PMID: 40869270] (Mouse) Mouse
Lorenzo-Anota, HY;Galván-Marroquín, JE;Sansén-Moreno, A;López-Morán, S;Benavides, J;Mayolo-Deloisa, K;Rito-Palomares, M;Lozano, O; Higher confluency enhances lipid droplet size, triglyceride content and endocrine function on white-like adipocytes Archives of biochemistry and biophysics 2025-08-02 [PMID: 40759356] (Mouse) Mouse
Hernández-Arciga, U;Stamenkovic, C;Yadav, S;Nicoletti, C;Albalawy, WN;Al Hammood, F;Gonzalez, TF;Naikwadi, MU;Graham, A;Smarz, C;Little, GJ;Williams, SG;McMahon, B;Sipula, IJ;Vandevender, AM;Chuan, B;Cooke, D;Pinto, AFM;Flores, LC;Hartman, HL;Diedrich, JK;Brooke, RT;Alder, JK;Frahm, KA;Pascal, LE;Stolt, E;Troensegaard, H;Øvrebø, B;Elshorbagy, A;Molina, E;Vinknes, KJ;Tan, RJ;Weisz, OA;Bueno, M;Eickelberg, O;Steinhauser, ML;Finkel, T;Ables, GP;Ikeno, Y;Olsen, T;Sacco, A;Jurczak, MJ;Sukoff Rizzo, SJ;Parkhitko, AA; Dietary methionine restriction started late in life promotes healthy aging in a sex-specific manner Science advances 2025-04-18 [PMID: 40238871] (Mouse) Mouse
Kim, A;Jung, S;Kim, Y;Jung, J;Lee, S;Lee, H;Kim, MJ;Park, JY;Hwang, EM;Lee, J; Novel function of TREK-1 in regulating adipocyte differentiation and lipid accumulation Cell death & disease 2025-03-08 [PMID: 40057491] (Mouse, Transgenic Mouse) Mouse, Transgenic Mouse
Du, T;Liu, S;Yu, H;Hu, T;Huang, L;Gao, L;Jia, L;Hu, J;Yu, Y;Sun, Q; Chronic sleep deprivation disturbs energy balance modulated by suprachiasmatic nucleus efferents in mice BMC biology 2024-12-23 [PMID: 39710657] (Mouse) Mouse
Li, S;Zhou, L;Ren, J;Zhang, Q;Xiao, X; Maternal exercise programs placental miR-495-5p-mediated Snx7 expression and kynurenic acid metabolic pathway induced by prenatal high-fat diet: based on miRNA-seq, transcriptomics, and metabolomics The Journal of nutritional biochemistry 2024-12-06 [PMID: 39647668] (Mouse) Mouse
Pomar, CA;Trepiana, J;Besné-Eseverri, I;Castillo, P;Palou, A;Palou, M;Portillo, MP;Picó, C; Maternal Dietary Improvement or Leptin Supplementation During Suckling Mitigates the Long-Term Impact of Maternal Obesogenic Conditions on Inflammatory and Oxidative Stress Biomarkers in the Offspring of Diet-Induced Obese Rats International journal of molecular sciences 2024-11-05 [PMID: 39595945] (Rat) Rat
Show All 205 Publications.

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Product General Protocols

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FAQs for Leptin/OB (MOB00B). (Showing 1 - 1 of 1 FAQs).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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