Mouse/Rat Leptin Quantikine ELISA Kit, 2 Plate

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      MOB00
    • Availability
      Product Discontinued

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Mouse/Rat Leptin Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse Leptin Immunoassay is a 4.5 hour solid phase ELISA designed to measure soluble mouse leptin in cell culture supernates, serum*, and plasma. It contains recombinant mouse leptin expressed in E. coli and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse leptin accurately. Results obtained measurin...g natural mouse leptin showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse leptin.

*Pregnant mouse serum from the second trimester of pregnancy through post-partum day 1 require acid/urea activation of mouse leptin.
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Specificity
Natural and recombinant mouse and rat Leptin.  This assay cross-reacts 0.24% with Human Leptin.
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Lep

Applications/Dilutions

Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using MOB00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat Leptin Quantikine ELISA Kit, 2 Plate

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor
  • OBOBS

Background

Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Leptin/OB (MOB00)(121)

We have publications tested in 3 confirmed species: Mouse, Rat, Hamster.


Filter By Application
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Filter By Species
Mouse
(89)
Rat
(30)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 121. Show All 121 Publications.
Publications using MOB00 Applications Species
N Kawao, Y Takafuji, M Ishida, K Okumoto, H Morita, M Muratani, H Kaji Roles of the vestibular system in obesity and impaired glucose metabolism in high-fat diet-fed mice PLoS ONE, 2020;15(2):e0228685. 2020 [PMID: 32012199] (Mouse) Mouse
S Hoshino, M Kobayashi, R Tagawa, R Konno, T Abe, K Furuya, K Miura, H Wakasawa, N Okita, Y Sudo, Y Mizunoe, Y Nakagawa, T Nakamura, H Kawabe, Y Higami WWP1 knockout in mice exacerbates obesity-related phenotypes in white adipose tissue but improves whole body glucose metabolism FEBS Open Bio, 2020;0(0):. 2020 [PMID: 31965758] (Mouse) Mouse
CI Gil, M Ost, J Kasch, S Schumann, S Heider, S Klaus Role of GDF15 in active lifestyle induced metabolic adaptations and acute exercise response in mice Sci Rep, 2019;9(1):20120. 2019 [PMID: 31882966] (Mouse) Mouse
N Kawao, M Ishida, H Kaji Roles of leptin in the recovery of muscle and bone by reloading after mechanical unloading in high fat diet-fed obese mice PLoS ONE, 2019;14(10):e0224403. 2019 [PMID: 31648235] (Mouse) Mouse
P Siriarchav, MC Kruger, MR Miller, HS Tian, FM Wolber The Preventive Effects of Greenshell Mussel (Perna canaliculus) on Early-Stage Metabolic Osteoarthritis in Rats with Diet-Induced Obesity Nutrients, 2019;11(7):. 2019 [PMID: 31311115] (Rat) Rat
C Gougoula, AP Bielfeld, SJ Pour, M Sager, JS Krüssel, WPM Benten, DM Baston-Büs Metabolic and behavioral parameters of mice with reduced expression of Syndecan-1 PLoS ONE, 2019;14(7):e0219604. 2019 [PMID: 31299063] (Mouse) Mouse
R Tazawa, K Uchida, H Fujimaki, M Miyagi, G Inoue, H Sekiguchi, K Murata, K Takata, A Kawakubo, M Takaso Elevated leptin levels induce inflammation through IL-6 in skeletal muscle of aged female rats BMC Musculoskelet Disord, 2019;20(1):199. 2019 [PMID: 31077169] (Rat) Rat
JA Deis, H Guo, Y Wu, C Liu, DA Bernlohr, X Chen Adipose Lipocalin 2 overexpression protects against age-related decline in thermogenic function of adipose tissue and metabolic deterioration Mol Metab, 2019;0(0):. 2019 [PMID: 30928474] (Mouse) Mouse
E ?ebrowska, M Maciejczyk, M ?endzian-P, A Zalewska, A Chabowski High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus Int J Mol Sci, 2019;20(7):. 2019 [PMID: 30925663] (Rat) Rat
M Pierard, A Tassin, S Conotte, K Zouaoui Bo, A Legrand Sustained Intermittent Hypoxemia Induces Adiponectin Oligomers Redistribution and a Tissue-Specific Modulation of Adiponectin Receptor in Mice Front Physiol, 2019;10(0):68. 2019 [PMID: 30800074] (Mouse) Mouse
Show All 121 Publications.

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Product General Protocols

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FAQs for Leptin/OB (MOB00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP MOB00B
HRP MOB00B
HRP MOB00B

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Bioinformatics

Gene Symbol Lep