MOG Antibody

Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MOG antibody [C2C3], C-term (NBP3-48458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

MOG antibody [C2C3], C-term detects MOG protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MOG protein stained by MOG antibody [C2C3], C-term (NBP3-48458) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] diluted at 1:500.Blue: Fluoroshield with DAPI .

MOG antibody [C2C3], C-term detects MOG protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: MOG protein stained by MOG antibody [C2C3], C-term (NBP3-48458) diluted at 1:250.Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MOG Antibody Summary

• Description: Novus Biologicals Rabbit MOG Antibody (NBP3-48458) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide encompassing a sequence within the Extracellular domain of human Myelin. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MOG Antibody

• MGC26137
• MOG Ig-AluB
• MOG
• MOGIG2
• myelin oligodendrocyte glycoprotein
• myelin-oligodendrocyte glycoprotein

Background

Myelin is produced by oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system (PNS). Defining the differences between CNS and PNS myelin may provide insights into the process of myelination and the pathogenesis of certain idiopathic diseases involving myelin such as multiple sclerosis, certain leukodystrophies, and other myelinopathies. Myelin is composed of about 80% lipid fat and about 20% protein. Some of the proteins that make up myelin are Myelin basic protein (MBP), Myelin oligodendrocyte glycoprotein (MOG) and Proteolipid protein (PLP). Myelin is made up primarily of a glycolipid called galactocerebroside. The intertwining of the hydrocarbon chains of sphingomyelin serve to strengthen the myelin sheath.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.