MMP-9 Antibody (4A3) - Azide and BSA Free

Western Blot: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855] - Identification of gelatinases by Western blot analysis. A pool of sera from 10 patients with inactive RR-MS was used. For 2-DZ, pooled sera were concentrated 10-fold and 40 ug of total proteins were applied (left panels). For Western blot analysis pooled sera were concentrated 40-fold and 160 ug of total proteins were subjected to 2-DE (right panels). 2-DE gels were cut and the corresponding nitrocellulose membranes were treated with anti-NGAL Ab (A'), anti-MMP-2 Ab (B') and Anti-MMP-9 (4A3) Ab, recognizing the 65 kD and the 82 kD activated forms (C'); Gel C show the region with isoelectric point between pH 4-5.5 and molecular mass between 85 and 55 kD, analysed by Western blot in C', respectively. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license. Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855] - Analysis of a FFPE section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary antibody and DAB reagent followed by hematoxylin counterstaining. This antibody clone generated an extra

Western Blot: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855] - 1.5 ul of serum from healthy control (HC) or from a patient with inactive RR-MS loaded on a 7.5% polyacrylamide gel copolymerized with 0.1% (w/v) gelatin (1-DZ). 2 ul of fivefold concentrated (Vivaspin 500, MWCO 50,000; GE Healthcare) serum samples (the same as in 1-DZ) separated on a 7.5% polyacrylamide gel without gelatin (1-DE), transferred on nitrocellulose membranes & incubated with the following primary antibodies: Anti-NGAL (5G5) at 2.0 ug/ml; Anti-MMP-9 (Ab-8) (IA5) at 2.66 ug/ml; Anti-MMP-2 (Ab-4) (75-7F7) at 1.0 ug/ml & Anti-MMP-9 Ab (4A3) at 2.0 ug/ml. Image collected & cropped by CiteAb from the following publication (null), licensed under a CC-BY license. Image from the st&ard format of this antibody.

Western Blot: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855] - Analysis of activated MMP9 expression in MMP9 proenzyme incubated with trypsin for various times. Image from the standard format of this antibody.

Immunohistochemistry: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855] - MMP-9 (green) was detected in murine brain (cortex) using MMP-9-DyLight 488 (4A3) with a concentration of 1:20 in PBS for 2 hours. MMP9-Signals are overlaped with the corresponding phase contrast image. Image from verified customer review. Image using the

Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855] - Analysis of a FFPE tissue section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary antibody and DAB reagent followed by hematoxylin counterstaining. This antibody generated a speci

CyTOF-ready: MMP-9 Antibody (4A3) - Azide and BSA Free [NBP2-80855] - Analysis of MMP-9 antibody on human tissue. MMP9 (red), nuclei (blue). Scale, 50um.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, CyTOF-ready
• Clone: 4A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

MMP-9 Antibody (4A3) - Azide and BSA Free Summary

• Immunogen: Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
• Localization: Secreted > extracellular space > extracellular matrix
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP9
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready reported by customer review
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:500-1:1000
• Application Notes: In Western blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30772382).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine (pH 7.5), 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for MMP-9 Antibody (4A3) - Azide and BSA Free

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

MMP9, also known as Matrix metallopeptidase 9 or gelatinase B is a member of the matrix metalloproteinase (MMP) family. The MMPs are zinc-containing enzymes involved in the degradation of the extracellular matrix in normal physiological processes including embryonic development, reproduction and tissue remodeling. Most MMP family members are secreted as inactive proproteins that become activated when cleaved by extracellular proteinases. The proenzyme MMP9 is thought to be a 92 kDa protein. Processing of the precursor MMP9 yields different active forms, two of which were identified in Duncan et al 1998 (PMID: 9851689) to be 82 kDa and 63 kDa proteins. The 82 kDa protein results from cleavage at the Arg87-Phe88 bond. And the 63 kDa protein is caused by a further cleavage to the 82 kDa protein within the C-terminus domain between Arg527-Gly528. The main 63 kDa protein produced thus has the N-terminus of activated MMP9 and has a cleaved C-terminus. Antibodies specific to the active forms of MMP9 can be useful tools for studying important pathological processes such as tumor invasion, metastasis and arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-9 Antibody (NBP2-80855)(2)

Publications using NBP2-80855

• Radnaa E, Richardson L, Goldman B Et al. Stress signaler p38 mitogen-activated kinase activation: a cause for concern? Clin Sci (Lond) 2022-10-17 [PMID: 36250628] (CyTOF-ready, Human)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.
• Gama Sosa MA, De Gasperi R, Pryor D et al. Low-level blast exposure induces chronic vascular remodeling, perivascular astrocytic degeneration and vascular-associated neuroinflammation Acta Neuropathol Commun 2021-10-15 [PMID: 34654480] (IF/IHC, Rat)
  
  Details:
  Citation using the Azide and BSA Free format of this antibody.

Review for MMP-9 Antibody (NBP2-80855) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Enkhtuya Radnaa CyTOF Human 01/04/2022

Summary

• Application: CyTOF
• Sample Tested: Human tissue origin
• Species: Human
• Lot: D-2

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

FAQs for MMP-9 Antibody (NBP2-80855). (Showing 1 - 1 of 1 FAQs).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Bioinformatics

• Gene Symbol: MMP9