MMP-9 Antibody (4A3) [FITC]

Images

 
Immunohistochemistry-Frozen: MMP-9 Antibody (4A3) [FITC] [NBP2-13173F] - MMP-9 (green) was detected in murine brain (cortex) using MMP-9-DyLight 488 (4A3) with a concentration of 1:20 in PBS for 2 hours. MMP9-Signals ...read more

Product Details

Summary
Reactivity Hu, Rt, Po, Ca, Eq, Pm, RbSpecies Glossary
Applications ELISA, IHC, IHC-Fr, IHC-P
Clone
4A3
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

MMP-9 Antibody (4A3) [FITC] Summary

Immunogen
Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Localization
Secreted > extracellular space > extracellular matrix
Specificity
Active MMP9. It does not react with the MMP9 proenzyme or the active and proenzyme forms of MMP2.
Predicted Species
Porcine (100%), Rabbit (100%), Canine (100%), Primate (100%), Equine (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MMP9
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This MMP9 Antibody (4A3) is useful for Western Blot, ELISA and Immunohistochemistry on paraffin embedded sections. In Western Blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. IHC-P from verified customer review.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-13173F in the following applications:

Reactivity Notes

Human and rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MMP-9 Antibody (4A3) [FITC]

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

MMP9, also known as Matrix metallopeptidase 9 or gelatinase B is a member of the matrix metalloproteinase (MMP) family. The MMPs are zinc-containing enzymes involved in the degradation of the extracellular matrix in normal physiological processes including embryonic development, reproduction and tissue remodeling. Most MMP family members are secreted as inactive proproteins that become activated when cleaved by extracellular proteinases. The proenzyme MMP9 is thought to be a 92 kDa protein. Processing of the precursor MMP9 yields different active forms, two of which were identified in Duncan et al 1998 (PMID: 9851689) to be 82 kDa and 63 kDa proteins. The 82 kDa protein results from cleavage at the Arg87-Phe88 bond. And the 63 kDa protein is caused by a further cleavage to the 82 kDa protein within the C-terminus domain between Arg527-Gly528. The main 63 kDa protein produced thus has the N-terminus of activated MMP9 and has a cleaved C-terminus. Antibodies specific to the active forms of MMP9 can be useful tools for studying important pathological processes such as tumor invasion, metastasis and arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Rt, Po, Ca, Eq, Pm, Rb
Applications: ELISA, IHC, IHC-Fr, IHC-P

Publications for MMP-9 Antibody (NBP2-13173F) (0)

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Review for MMP-9 Antibody (NBP2-13173F) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-13173F:
Filter by Applications
IHC-Fr
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen MMP-9 NBP2-13173F
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reviewed by:
Christian Ostalecki
IHC-Fr Mouse 05/30/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain (cortex) tissue
SpeciesMouse
LotA-3-020116-F

Product General Protocols

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FAQs for MMP-9 Antibody (NBP2-13173F). (Showing 1 - 1 of 1 FAQ).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP2-13173B
DyLight 405 Labeled NBP2-13173V
DyLight 488 Labeled NBP2-13173G
DyLight 550 Labeled NBP2-13173R
DyLight 650 Labeled NBP2-13173C
HRP Labeled NBP2-13173H

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Bioinformatics Tool for MMP-9 Antibody (NBP2-13173F)

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Blogs on MMP-9.

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Recent Reviews

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Christian Ostalecki
05/30/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol MMP9