MMP-9 Antibody (4A3) [HRP] Summary
Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Secreted > extracellular space > extracellular matrix
Active MMP9. It does not react with the MMP9 proenzyme or the active and proenzyme forms of MMP2.
Porcine (100%), Rabbit (100%), Primate (100%), Equine (100%), Canine (100%). Backed by our 100% Guarantee.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot
This MMP9 Antibody (4A3) is useful for Western Blot, ELISA and Immunohistochemistry on paraffin embedded sections. In Western Blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Packaging, Storage & Formulations
Store at 4C in the dark.
Protein G purified
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for MMP-9 Antibody (4A3) [HRP]
- 92 kDa gelatinase
- 92 kDa type IV collagenase
- EC 3.4.24
- EC 22.214.171.124
- Gelatinase B
- macrophage gelatinase
- matrix metallopeptidase 9
- matrix metalloproteinase 9
- matrix metalloproteinase-9
- type V collagenase
MMP9, also known as Matrix metallopeptidase 9 or gelatinase B is a member of the matrix metalloproteinase (MMP) family. The MMPs are zinc-containing enzymes involved in the degradation of the extracellular matrix in normal physiological processes including embryonic development, reproduction and tissue remodeling. Most MMP family members are secreted as inactive proproteins that become activated when cleaved by extracellular proteinases. The proenzyme MMP9 is thought to be a 92 kDa protein. Processing of the precursor MMP9 yields different active forms, two of which were identified in Duncan et al 1998 (PMID: 9851689) to be 82 kDa and 63 kDa proteins. The 82 kDa protein results from cleavage at the Arg87-Phe88 bond. And the 63 kDa protein is caused by a further cleavage to the 82 kDa protein within the C-terminus domain between Arg527-Gly528. The main 63 kDa protein produced thus has the N-terminus of activated MMP9 and has a cleaved C-terminus. Antibodies specific to the active forms of MMP9 can be useful tools for studying important pathological processes such as tumor invasion, metastasis and arthritis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
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FAQs for MMP-9 Antibody (NBP2-13173H). (Showing 1 - 1 of 1 FAQ).
I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any?
- We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)
Positive Control Lysate(s)
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