MMP-9 Antibody (4A3)


Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of activated MMP9 expression in MMP9 proenzyme incubated with trypsin for various times.
Immunohistochemistry: MMP-9 Antibody (4A3) [NBP2-13173] - MMP-9 (green) was detected in murine brain (cortex) using MMP-9-DyLight 488 (4A3) with a concentration of 1:20 in PBS for 2 hours. MMP9-Signals are overlaped more
Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE tissue section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled more
Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary more

Product Details

Reactivity Hu, Rt, Po, Ca, Eq, Pm, RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
1.0 mg/ml

MMP-9 Antibody (4A3) Summary

Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Secreted > extracellular space > extracellular matrix
Active MMP9. It does not react with the MMP9 proenzyme or the active and proenzyme forms of MMP2.
Predicted Species
Porcine (100%), Rabbit (100%), Primate (100%), Equine (100%), Canine (100%). Backed by our 100% Guarantee.
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500 - 1:1000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
Application Notes
In Western blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
MMP-9 Lysate (NBP2-66211)
Reviewed Applications
Read 2 Reviews rated 3.5
NBP2-13173 in the following applications:

Read Publications using
NBP2-13173 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    6 publications

Reactivity Notes

Human and rat.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine (pH 7.5), 0.15 M NaCl
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for MMP-9 Antibody (4A3)

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase


MMP9, also known as Matrix metallopeptidase 9 or gelatinase B is a member of the matrix metalloproteinase (MMP) family. The MMPs are zinc-containing enzymes involved in the degradation of the extracellular matrix in normal physiological processes including embryonic development, reproduction and tissue remodeling. Most MMP family members are secreted as inactive proproteins that become activated when cleaved by extracellular proteinases. The proenzyme MMP9 is thought to be a 92 kDa protein. Processing of the precursor MMP9 yields different active forms, two of which were identified in Duncan et al 1998 (PMID: 9851689) to be 82 kDa and 63 kDa proteins. The 82 kDa protein results from cleavage at the Arg87-Phe88 bond. And the 63 kDa protein is caused by a further cleavage to the 82 kDa protein within the C-terminus domain between Arg527-Gly528. The main 63 kDa protein produced thus has the N-terminus of activated MMP9 and has a cleaved C-terminus. Antibodies specific to the active forms of MMP9 can be useful tools for studying important pathological processes such as tumor invasion, metastasis and arthritis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IP
Species: Hu, Rb
Applications: WB, Flow, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Rt, Po, Ca, Eq, Pm, Rb
Applications: WB, ELISA, IHC, IHC-P

Publications for MMP-9 Antibody (NBP2-13173)(9)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: IHC, IHC-P, WB.

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Showing Publications 1 - 9 of 9.
Publications using NBP2-13173 Applications Species
van der Valk DC, van der Ven CFT, Blaser MC et al. Engineering a 3D-Bioprinted Model of Human Heart Valve Disease Using Nanoindentation-Based Biomechanics Nanomaterials (Basel) May 3 2018 [PMID: 29751516] (Human) Human
Liao W, Fan H, Wu J. Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Inhibits Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Angiotensin Type I Receptor J. Agric. Food Chem. May 23 2018 [PMID: 29714061] (WB, Rat) WB Rat
Svalina MN, Kikuchi K, Abraham J et al. IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma. Sci Rep 2016 Jun 03 [PMID: 27255663] (WB) WB
Cavkaytar S, Tapisiz OL, Kiykac Altinbas S et al. Clarithromycin regresses endometriotic implants in rat endometriosis model. J Obstet Gynaecol 2015 Jul 08 [PMID: 26156575]
Goktolga U, Cavkaytar S, Altinbas SK et al. Effect of the non-specific matrix metalloproteinase inhibitor Doxycycline on endometriotic implants in an experimental rat model. Exp Ther Med 2015 May [PMID: 26136898] (IHC, Rat) IHC Rat
Rossano R, Larocca M, Riviello L et al. Heterogeneity of serum gelatinases MMP-2 and MMP-9 isoforms and charge variants. J Cell Mol Med. [PMID: 24616914] (WB, Human)

MMP-9 antibody (clone 4A3) was used at 2ug/ml concentration for WB analysis of 1.5ul serum from healthy control or from a patient with inactive relapsing-remitting multiple sclerosis (RR-MS). The antibody was also used for 2-D zymography (2-DZ) analysis o
WB Human
Duncan ME, Richardson JP, Murray et al. Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated Eur J Biochem. 1998 Nov 15 [PMID: 9851689] (WB, IHC-P, Human) WB, IHC-P Human
Cirella K, Blennerhassett M. Inflammation Promotes the Processing of Glial Cell-Line Derived Neurotrophic Factor by Matrix Metalloproteinase-9 in Intestinal Smooth Muscle Cells thesis Feb 1 2016 (WB, Human, Rat) WB Human, Rat
Ferrer CM, Lu TY, Bacigalupa ZA et al. O-GlcNAcylation regulates breast cancer metastasis via SIRT1 modulation of FOXM1 pathway Oncogene 2016 Jun 27 [PMID: 27345396] (WB) WB

Reviews for MMP-9 Antibody (NBP2-13173) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using NBP2-13173:
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Images Ratings Applications Species Date Details
Western Blot MMP-9 NBP2-13173
reviewed by:
WB Rat 07/20/2016


ApplicationWestern Blot
Sample Testedrat smooth muscle


Blocking Details1hr block in 2% milk TBS, 0.1% Tween-20

Primary Anitbody

Dilution Ratioprimary 1/750 in blocking buffer fridge overnight

Secondary Antibody

Secondary Descriptiongoat anti-mouse HRP in TBS-T 1 hr room temp
Secondary Manufacturer Cat#Fisher 31430
Secondary Concentration1 in 20,000


Detection NotesBioRad GelDoc
Western Blot MMP-9 NBP2-13173
reviewed by:
Kristina Knubel
WB Human 02/25/2014


ApplicationWestern Blot
Sample TestedU251 glioma cell line; whole cell lysate


Blocking Details5% milk in TBST, 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:1000 MMP9 (4A3), overnight at 4 degrees, rocking, in 5% milk in TBST.

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat##170-6516; Biorad
Secondary Concentration1:4000


Detection Notes3 min incubation with ECL-Plus. Exposed to radiograph film for 5 min. Developed in a standard x-ray developer.

Product General Protocols

View specific protocols for MMP-9 Antibody (NBP2-13173): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MMP-9 Antibody (NBP2-13173). (Showing 1 - 2 of 2 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA. looks like 2 have been tested for capture (please note the tested species for each of these). has been tested for detection.
  2. Would it be possible for you to send me your validation data on MMP9 Antibody IIA5 NBP1-40248? I am considering using it in my research project on colorectal cancer liver metastases.
    • Our MMP9 Antibody IIA5 NBP1-40248 was validated in Western blot through external validation, and unfortunately, I do not have an images to share at the moment. NBP2-13173 is another very similar monoclonal antibody validated in human and rat for use in Western blot. We do have images from in-house testing for this product (please see the datasheet for NBP2-13173, under the images tab), so you may be more comfortable ordering this antibody for this target.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-13173AF405
Alexa Fluor 488 Labeled NBP2-13173AF488
Alexa Fluor 647 Labeled NBP2-13173AF647
Alexa Fluor 700 Labeled NBP2-13173AF700
Biotin Labeled NBP2-13173B
DyLight 405 Labeled NBP2-13173V
DyLight 488 Labeled NBP2-13173G
DyLight 550 Labeled NBP2-13173R
DyLight 650 Labeled NBP2-13173C
FITC Labeled NBP2-13173F
HRP Labeled NBP2-13173H

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Blogs on MMP-9.

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Recent Reviews


Application: WB
Species: Rat

Kristina Knubel
Application: WB
Species: Human


Gene Symbol MMP9