MMP-9 Antibody (4A3)

Images

 
Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of activated MMP9 expression in MMP9 proenzyme incubated with trypsin for various times.
Immunohistochemistry: MMP-9 Antibody (4A3) [NBP2-13173] - MMP-9 (green) was detected in murine brain (cortex) using MMP-9-DyLight 488 (4A3) with a concentration of 1:20 in PBS for 2 hours. MMP9-Signals are overlaped ...read more
Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - Identification of gelatinases by Western blot analysis. A pool of sera from 10 patients with inactive RR-MS was used. For 2-DZ, pooled sera were concentrated 10-fold and ...read more
Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - 1-DZ analysis and identification of gelatinases by Western blot. 1.5 ul of serum from healthy control (HC) or from a patient with inactive RR-MS were loaded on a 7.5% ...read more
Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE tissue section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled ...read more
Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Eq, Pm, RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
4A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MMP-9 Antibody (4A3) Summary

Immunogen
Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Localization
Secreted > extracellular space > extracellular matrix
Specificity
Active MMP9. It does not react with the MMP9 proenzyme or the active and proenzyme forms of MMP2.
Predicted Species
Porcine (100%), Rabbit (100%), Primate (100%), Canine (100%), Equine (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MMP9
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
In Western blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control
MMP-9 Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 3.5
using
NBP2-13173 in the following applications:

Publications
Read Publications using
NBP2-13173 in the following applications:

  • IHC
    2 publications
  • 3 publications
  • WB
    11 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32725911). Human and rat. Mouse reactivity reported in scientific literature (PMID: 30772382)..

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine (pH 7.5), 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MMP-9 Antibody (4A3)

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

MMP9, also known as Matrix metallopeptidase 9 or gelatinase B is a member of the matrix metalloproteinase (MMP) family. The MMPs are zinc-containing enzymes involved in the degradation of the extracellular matrix in normal physiological processes including embryonic development, reproduction and tissue remodeling. Most MMP family members are secreted as inactive proproteins that become activated when cleaved by extracellular proteinases. The proenzyme MMP9 is thought to be a 92 kDa protein. Processing of the precursor MMP9 yields different active forms, two of which were identified in Duncan et al 1998 (PMID: 9851689) to be 82 kDa and 63 kDa proteins. The 82 kDa protein results from cleavage at the Arg87-Phe88 bond. And the 63 kDa protein is caused by a further cleavage to the 82 kDa protein within the C-terminus domain between Arg527-Gly528. The main 63 kDa protein produced thus has the N-terminus of activated MMP9 and has a cleaved C-terminus. Antibodies specific to the active forms of MMP9 can be useful tools for studying important pathological processes such as tumor invasion, metastasis and arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MMP200
Species: Hu, Mu, Rt, Po, Ca
NBP1-47842
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P, IP
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-56583
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
NBP2-67415
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-50037
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, KO

Publications for MMP-9 Antibody (NBP2-13173)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-P
(3)
WB
(11)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
Rat
(8)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NBP2-13173 Applications Species
Chen G, Ge D, Zhu B et al. Salvia miltiorrhiza Injection Alleviates LPS-Induced Acute Lung Injury by Adjusting the Balance of MMPs/TIMPs Ratio Evidence-Based Complementary and Alternative Medicine Jul 20 2020 [PMID: 32765635] (WB, Rat) WB Rat
Ohene-Nyako M, Persons AL, Napier TC Hippocampal blood-brain barrier of methamphetamine self-administering HIV-1 transgenic rats Eur. J. Neurosci. Jul 29 2020 [PMID: 32725911] (Rat) Rat
Fisch S, Bachner-Hinenzon N, Ertracht O et al. Localized Antileptin Therapy Prevents Aortic Root Dilatation and Preserves Left Ventricular Systolic Function in a Murine Model of Marfan Syndrome J Am Heart Assoc May 18 2020 [PMID: 32378446] (IHC-P, Mouse) IHC-P Mouse
Chen G, Ge D, Zhu B et al. Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury J. Int. Med. Res. Apr 1 2020 [PMID: 32339071] (WB, Rat) WB Rat
Retana-Lobo C, Guerreiro-Tanomaru Jm, Tanomaru-Filho M et Al. Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin Materials (Basel) Feb 27 2020 [PMID: 32120926] (IHC-P, Human) IHC-P Human
Liao W, Fan H, Davidge ST, Wu J Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Reduces Blood Pressure in Spontaneously Hypertensive Rats via the ACE2/Ang (1-7)/Mas Receptor Axis Mol Nutr Food Res May 1 2019 [PMID: 30913349] (WB, Rat) WB Rat
Ducas A, Kuhn D, Bath L et al. Increased MMP-9 activity correlates with flow-mediated intraluminal thrombus deposition and wall degeneration in human AAA. JVS: Vascular Science Oct 1 2020 (IHC, Human) IHC Human
Zoumboulakis D, Cirella KR, Gougeon PY et al. MMP-9 Processing of Intestinal Smooth Muscle-derived GDNF is Required for Neurotrophic Action on Enteric Neurons Neuroscience Jul 16 2020 [PMID: 32682824] (WB, Rat) WB Rat
Lobanovskaya N, Zharkovsky A. A role of PSA-NCAM in the survival of retinal ganglion cells (RGCs) after kainic acid damage Neurotoxicology Feb 14 2019 [PMID: 30772382] (WB, Mouse) WB Mouse
Liao W, Fan H, Wu J. Egg White-Derived Antihypertensive Peptide IRW (Ile-Arg-Trp) Inhibits Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Angiotensin Type I Receptor J. Agric. Food Chem. May 23 2018 [PMID: 29714061] (WB, Rat) WB Rat
Show All 18 Publications.

Reviews for MMP-9 Antibody (NBP2-13173) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using NBP2-13173:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MMP-9 NBP2-13173
Enlarge
4
reviewed by:
Anonymous
WB Rat 07/20/2016
View

Summary

ApplicationWestern Blot
Sample Testedrat smooth muscle
SpeciesRat
LotA-2

Blocking

Blocking Details1hr block in 2% milk TBS, 0.1% Tween-20

Primary Anitbody

Dilution Ratioprimary 1/750 in blocking buffer fridge overnight

Secondary Antibody

Secondary Descriptiongoat anti-mouse HRP in TBS-T 1 hr room temp
Secondary Manufacturer Cat#Fisher 31430
Secondary Concentration1 in 20,000

Details

Detection NotesBioRad GelDoc
Western Blot MMP-9 NBP2-13173
Enlarge
3
reviewed by:
Kristina Knubel
WB Human 02/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedU251 glioma cell line; whole cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in TBST, 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:1000 MMP9 (4A3), overnight at 4 degrees, rocking, in 5% milk in TBST.

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat##170-6516; Biorad
Secondary Concentration1:4000

Details

Detection Notes3 min incubation with ECL-Plus. Exposed to radiograph film for 5 min. Developed in a standard x-ray developer.

Product General Protocols

View specific protocols for MMP-9 Antibody (NBP2-13173): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MMP-9 Antibody (NBP2-13173). (Showing 1 - 2 of 2 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)
  2. Would it be possible for you to send me your validation data on MMP9 Antibody IIA5 NBP1-40248? I am considering using it in my research project on colorectal cancer liver metastases.
    • Our MMP9 Antibody IIA5 NBP1-40248 was validated in Western blot through external validation, and unfortunately, I do not have an images to share at the moment. NBP2-13173 is another very similar monoclonal antibody validated in human and rat for use in Western blot. We do have images from in-house testing for this product (please see the datasheet for NBP2-13173, under the images tab), so you may be more comfortable ordering this antibody for this target.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-13173AF350
Alexa Fluor 405 NBP2-13173AF405
Alexa Fluor 488 NBP2-13173AF488
Alexa Fluor 532 NBP2-13173AF532
Alexa Fluor 594 NBP2-13173AF594
Alexa Fluor 647 NBP2-13173AF647
Alexa Fluor 700 NBP2-13173AF700
Alexa Fluor 750 NBP2-13173AF750
Biotin NBP2-13173B
DyLight 350 NBP2-13173UV
DyLight 405 NBP2-13173V
DyLight 488 NBP2-13173G
DyLight 550 NBP2-13173R
DyLight 594 NBP2-13173DL594
DyLight 650 NBP2-13173C
DyLight 680 NBP2-13173FR
DyLight 755 NBP2-13173IR
FITC NBP2-13173F
HRP NBP2-13173H
Janelia Fluor 549 NBP2-13173JF549
Janelia Fluor 646 NBP2-13173JF646

Additional MMP-9 Products

Bioinformatics Tool for MMP-9 Antibody (NBP2-13173)

Discover related pathways, diseases and genes to MMP-9 Antibody (NBP2-13173). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MMP-9 Antibody (NBP2-13173)

Discover more about diseases related to MMP-9 Antibody (NBP2-13173).
 

Pathways for MMP-9 Antibody (NBP2-13173)

View related products by pathway.

PTMs for MMP-9 Antibody (NBP2-13173)

Learn more about PTMs related to MMP-9 Antibody (NBP2-13173).
 

Research Areas for MMP-9 Antibody (NBP2-13173)

Find related products by research area.

Blogs on MMP-9.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Anonymous
07/20/2016
Application: WB
Species: Rat

Kristina Knubel
02/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MMP9
Entrez
Uniprot