MMP-9 Antibody (4A3)

Images

 
Western Blot: MMP9 Antibody (4A3) [NBP2-13173] - Western blot analysis of activated MMP9 expression in MMP9 proenzyme incubated with trypsin for various times.
Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200 dilution. The ...read more
Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200 dilution. The ...read more

Product Details

Summary
Reactivity Hu, Rt, Po, Ca, Eq, Pm, RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
4A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MMP-9 Antibody (4A3) Summary

Immunogen
Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Localization
Secreted > extracellular space > extracellular matrix
Specificity
Active MMP9. It does not react with the MMP9 proenzyme or the active and proenzyme forms of MMP2.
Predicted Species
Porcine (100%), Rabbit (100%), Primate (100%), Equine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MMP9
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
This MMP9 Antibody (4A3) is useful for Western Blot, ELISA and Immunohistochemistry on paraffin embedded sections. In Western Blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Positive Control
MMP-9 Lysate (NBP2-66211)
Reviewed Applications
Read 2 Reviews rated 3.5
using
NBP2-13173 in the following applications:

Publications
Read Publications using
NBP2-13173 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    5 publications

Reactivity Notes

Human and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine (pH 7.5) and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MMP-9 Antibody (4A3)

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

MMP9, also known as Matrix metallopeptidase 9 or gelatinase B is a member of the matrix metalloproteinase (MMP) family. The MMPs are zinc-containing enzymes involved in the degradation of the extracellular matrix in normal physiological processes including embryonic development, reproduction and tissue remodeling. Most MMP family members are secreted as inactive proproteins that become activated when cleaved by extracellular proteinases. The proenzyme MMP9 is thought to be a 92 kDa protein. Processing of the precursor MMP9 yields different active forms, two of which were identified in Duncan et al 1998 (PMID: 9851689) to be 82 kDa and 63 kDa proteins. The 82 kDa protein results from cleavage at the Arg87-Phe88 bond. And the 63 kDa protein is caused by a further cleavage to the 82 kDa protein within the C-terminus domain between Arg527-Gly528. The main 63 kDa protein produced thus has the N-terminus of activated MMP9 and has a cleaved C-terminus. Antibodies specific to the active forms of MMP9 can be useful tools for studying important pathological processes such as tumor invasion, metastasis and arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-193
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF1310
Species: Hu
Applications: WB, IHC, IP
NB100-56583
Species: Hu
Applications: WB, Flow, IHC-P
MAB9181
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready
NB110-75039
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for MMP-9 Antibody (NBP2-13173)(7)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(1)
WB
(5)
All Applications
Filter By Species
Human
(3)
Rat
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP2-13173 Applications Species
Svalina MN, Kikuchi K, Abraham J et al. IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma. Sci Rep 2016 Jun 03 [PMID: 27255663] (WB) WB
Cavkaytar S, Tapisiz OL, Kiykac Altinbas S et al. Clarithromycin regresses endometriotic implants in rat endometriosis model. J Obstet Gynaecol 2015 Jul 08 [PMID: 26156575]
Goktolga U, Cavkaytar S, Altinbas SK et al. Effect of the non-specific matrix metalloproteinase inhibitor Doxycycline on endometriotic implants in an experimental rat model. Exp Ther Med 2015 May [PMID: 26136898] (IHC, Rat) IHC Rat
Rossano R, Larocca M, Riviello L et al. Heterogeneity of serum gelatinases MMP-2 and MMP-9 isoforms and charge variants. J Cell Mol Med. [PMID: 24616914] (WB, Human)

Details:
MMP-9 antibody (clone 4A3) was used at 2ug/ml concentration for WB analysis of 1.5ul serum from healthy control or from a patient with inactive relapsing-remitting multiple sclerosis (RR-MS). The antibody was also used for 2-D zymography (2-DZ) analysis o
WB Human
Duncan ME, Richardson JP, Murray et al. Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated Eur J Biochem. 1998 Nov 15 [PMID: 9851689] (WB, IHC-P, Human) WB, IHC-P Human
Cirella K, Blennerhassett M. Inflammation Promotes the Processing of Glial Cell-Line Derived Neurotrophic Factor by Matrix Metalloproteinase-9 in Intestinal Smooth Muscle Cells thesis Feb 1 2016 (WB, Human, Rat) WB Human, Rat
Ferrer CM, Lu TY, Bacigalupa ZA et al. O-GlcNAcylation regulates breast cancer metastasis via SIRT1 modulation of FOXM1 pathway Oncogene 2016 Jun 27 [PMID: 27345396] (WB) WB

Reviews for MMP-9 Antibody (NBP2-13173) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using NBP2-13173:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MMP-9 NBP2-13173
Enlarge
reviewed by:
Anonymous
WB Rat 07/20/2016
View

Summary

ApplicationWestern Blot
Sample Testedrat smooth muscle
SpeciesRat
LotA-2

Blocking

Blocking Details1hr block in 2% milk TBS, 0.1% Tween-20

Primary Anitbody

Dilution Ratioprimary 1/750 in blocking buffer fridge overnight

Secondary Antibody

Secondary Descriptiongoat anti-mouse HRP in TBS-T 1 hr room temp
Secondary Manufacturer Cat#Fisher 31430
Secondary Concentration1 in 20,000

Details

Detection NotesBioRad GelDoc
Western Blot MMP-9 NBP2-13173
Enlarge
reviewed by:
Kristina Knubel
WB Human 02/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedU251 glioma cell line; whole cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in TBST, 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:1000 MMP9 (4A3), overnight at 4 degrees, rocking, in 5% milk in TBST.

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, HRP
Secondary Manufacturer Cat##170-6516; Biorad
Secondary Concentration1:4000

Details

Detection Notes3 min incubation with ECL-Plus. Exposed to radiograph film for 5 min. Developed in a standard x-ray developer.

Product General Protocols

View specific protocols for MMP-9 Antibody (NBP2-13173): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MMP-9 Antibody (NBP2-13173). (Showing 1 - 2 of 2 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)
  2. Would it be possible for you to send me your validation data on MMP9 Antibody IIA5 NBP1-40248? I am considering using it in my research project on colorectal cancer liver metastases.
    • Our MMP9 Antibody IIA5 NBP1-40248 was validated in Western blot through external validation, and unfortunately, I do not have an images to share at the moment. NBP2-13173 is another very similar monoclonal antibody validated in human and rat for use in Western blot. We do have images from in-house testing for this product (please see the datasheet for NBP2-13173, under the images tab), so you may be more comfortable ordering this antibody for this target.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-13173AF405
Alexa Fluor 488 Labeled NBP2-13173AF488
Alexa Fluor 647 Labeled NBP2-13173AF647
Alexa Fluor 700 Labeled NBP2-13173AF700
Biotin Labeled NBP2-13173B
DyLight 405 Labeled NBP2-13173V
DyLight 488 Labeled NBP2-13173G
DyLight 550 Labeled NBP2-13173R
DyLight 650 Labeled NBP2-13173C
FITC Labeled NBP2-13173F
HRP Labeled NBP2-13173H

Additional Array Products

Bioinformatics Tool for MMP-9 Antibody (NBP2-13173)

Discover related pathways, diseases and genes to MMP-9 Antibody (NBP2-13173). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MMP-9 Antibody (NBP2-13173)

Discover more about diseases related to MMP-9 Antibody (NBP2-13173).
 

Pathways for MMP-9 Antibody (NBP2-13173)

View related products by pathway.

PTMs for MMP-9 Antibody (NBP2-13173)

Learn more about PTMs related to MMP-9 Antibody (NBP2-13173).
 

Research Areas for MMP-9 Antibody (NBP2-13173)

Find related products by research area.

Blogs on MMP-9.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Anonymous
07/20/2016
Application: WB
Species: Rat

Kristina Knubel
02/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MMP9
Entrez
Uniprot