MMP-9 Antibody (4A3) [DyLight 550]


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Product Details

Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

MMP-9 Antibody (4A3) [DyLight 550] Summary

Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Secreted > extracellular space > extracellular matrix
Active MMP9. It does not react with the MMP9 proenzyme or the active and proenzyme forms of MMP2.
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 550
DyLight 550 (A=562, E=576)
50mM Sodium Borate
0.05% Sodium Azide
Protein G purified


  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
This MMP9 Antibody (4A3) is useful for Western Blot, ELISA and Immunohistochemistry on paraffin embedded sections. In Western Blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and rat.

Alternate Names for MMP-9 Antibody (4A3) [DyLight 550]

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase


MMP9, also known as Matrix metallopeptidase 9 or gelatinase B is a member of the matrix metalloproteinase (MMP) family. The MMPs are zinc-containing enzymes involved in the degradation of the extracellular matrix in normal physiological processes including embryonic development, reproduction and tissue remodeling. Most MMP family members are secreted as inactive proproteins that become activated when cleaved by extracellular proteinases. The proenzyme MMP9 is thought to be a 92 kDa protein. Processing of the precursor MMP9 yields different active forms, two of which were identified in Duncan et al 1998 (PMID: 9851689) to be 82 kDa and 63 kDa proteins. The 82 kDa protein results from cleavage at the Arg87-Phe88 bond. And the 63 kDa protein is caused by a further cleavage to the 82 kDa protein within the C-terminus domain between Arg527-Gly528. The main 63 kDa protein produced thus has the N-terminus of activated MMP9 and has a cleaved C-terminus. Antibodies specific to the active forms of MMP9 can be useful tools for studying important pathological processes such as tumor invasion, metastasis and arthritis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NBP2-13173B
DyLight 405 Labeled NBP2-13173V
DyLight 488 Labeled NBP2-13173G
DyLight 650 Labeled NBP2-13173C
FITC Labeled NBP2-13173F
HRP Labeled NBP2-13173H

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Contact Information

Product PDFs


Gene Symbol MMP9

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