MMP-9 Antibody (4A3) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Rt, Po, Ca, Eq, Pm, RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
4A3
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

MMP-9 Antibody (4A3) [DyLight 488] Summary

Immunogen
Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Localization
Secreted > extracellular space > extracellular matrix
Specificity
Active MMP9. It does not react with the MMP9 proenzyme or the active and proenzyme forms of MMP2.
Predicted Species
Porcine (100%), Rabbit (100%), Primate (100%), Canine (100%), Equine (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MMP9
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This MMP9 Antibody (4A3) is useful for Western Blot, ELISA and Immunohistochemistry on paraffin embedded sections. In Western Blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MMP-9 Antibody (4A3) [DyLight 488]

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

MMP9, also known as Matrix metallopeptidase 9 or gelatinase B is a member of the matrix metalloproteinase (MMP) family. The MMPs are zinc-containing enzymes involved in the degradation of the extracellular matrix in normal physiological processes including embryonic development, reproduction and tissue remodeling. Most MMP family members are secreted as inactive proproteins that become activated when cleaved by extracellular proteinases. The proenzyme MMP9 is thought to be a 92 kDa protein. Processing of the precursor MMP9 yields different active forms, two of which were identified in Duncan et al 1998 (PMID: 9851689) to be 82 kDa and 63 kDa proteins. The 82 kDa protein results from cleavage at the Arg87-Phe88 bond. And the 63 kDa protein is caused by a further cleavage to the 82 kDa protein within the C-terminus domain between Arg527-Gly528. The main 63 kDa protein produced thus has the N-terminus of activated MMP9 and has a cleaved C-terminus. Antibodies specific to the active forms of MMP9 can be useful tools for studying important pathological processes such as tumor invasion, metastasis and arthritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP2-13173B
DyLight 405 Labeled NBP2-13173V
DyLight 550 Labeled NBP2-13173R
DyLight 650 Labeled NBP2-13173C
FITC Labeled NBP2-13173F
HRP Labeled NBP2-13173H

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Bioinformatics

Gene Symbol MMP9
Entrez