MMP-12 Antibody (703D10) - Azide and BSA Free

Immunohistochemistry: MMP-12 Antibody (703D10) [DDX0283P-100] - Staining of human lung cancer frozen section.

Flow Cytometry: MMP-12 Antibody (703D10) [DDX0283P-100] - Intracellular staining of canine cells

ELISA: MMP-12 Antibody (703D10) [DDX0283P-100] - HumanHME-MMP12 ELISA assay

• Reactivity: Hu, Ca
• Applications: ELISA, Flow, IHC
• Clone: 703D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

MMP-12 Antibody (703D10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse MMP-12 Antibody (703D10) - Azide and BSA Free (DDX0283P-100) is a monoclonal antibody validated for use in IHC, ELISA and Flow. Anti-MMP-12 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: MMP-12/HME was used as immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP12
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin

Reactivity Notes

Human, Canine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for MMP-12 Antibody (703D10) - Azide and BSA Free

• EC 3.4.24
• hME
• HMEEC 3.4.24.65
• Macrophage elastase
• macrophage metalloelastase
• matrix metallopeptidase 12 (macrophage elastase)
• matrix metalloproteinase 12 (macrophage elastase)
• Matrix metalloproteinase-12
• ME
• MGC138506
• MME
• MMP12
• MMP-12

Background

Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-12 Antibody (DDX0283P-100)(2)

Publications using DDX0283P-100

• Bonnefont-Rebeix C et al. Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology. Immunobiology 2016-01-01 [PMID: 26345430]
• Demets IK et al. Elevated MMP-12 protein levels in induced sputum from patients with COPD. Thorax 2006-03-01 [PMID: 16308335]

Bioinformatics

• Gene Symbol: MMP12