MLF1 Interacting Protein Antibody - BSA Free

Immunohistochemistry: MLF1 Interacting Protein Antibody - BSA Free [NBP3-45908] - Immunohistochemistry of paraffin-embedded human testis tissue slide using (MLF1 Interacting Protein Antibody) at dilution of 1:50

Western Blot: MLF1 Interacting Protein Antibody - BSA Free [NBP3-45908] - HepG2 cells were subjected to SDS PAGE followed by western blot with (MLF1 Interacting Protein antibody) at dilution of 1:500

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MLF1 Interacting Protein Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MLF1 Interacting Protein Antibody - BSA Free (NBP3-45908) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: MLF1 interacting protein. (Uniprot# Q71F23)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CENPU
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Application Notes: Theoretical Molecular Weight is 46-48 kDa

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MLF1 Interacting Protein Antibody - BSA Free

• CENP-50
• CENP-U
• CENPU50
• CENPUMLF1-interacting protein
• centromere protein of 50 kDa
• centromere protein U
• FLJ23468
• ICEN24
• Interphase centromere complex protein 24
• KLIP1CENP50
• KSHV latent nuclear antigen interacting protein 1
• KSHV latent nuclear antigen-interacting protein 1
• MLF1 interacting protein
• PBIP1
• Polo-box-interacting protein 1

Background

Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/ anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CENPU