Recombinant Human BMP-15 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human BMP-15 Protein Summary

Details of Functionality
Measured by its ability to induce Smad3 phosphorylation in P19 mouse embryonal carcinoma cells. Approximately 10 ng/mL can effectively induce Smad3 phosphorylation.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-15/GDF-9B protein
Gln268-Arg392 (Thr266Arg)
Accession #
N-terminal Sequence
Gln268
Protein/Peptide Type
Recombinant Proteins
Gene
BMP15
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
15 kDa, reducing conditions
Publications
Read Publications using
5096-BM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4  mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-15 Protein

  • BMP15
  • BMP-15
  • bone morphogenetic protein 15
  • GDF-9B
  • GDF9BODG2
  • Growth/differentiation factor 9B
  • POF4

Background

Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a TGF-beta superfamily ligand that is expressed by oocytes throughout folliculogenesis, and plays an important role in oocyte development (1). BMP-15 promotes the FSH‑independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis (2‑4). It also induces GC production of stem cell factor which, in turn, negatively regulates BMP-15 expression in oocytes (5). BMP-15 blocks the FSH‑induced GC expression of FSH R and multiple steroidogenic molecules (6). BMP-15 is synthesized with a 249 amino acid (aa) N-terminal propeptide (7). The propeptide is cleaved intracellularly from the 50 kDa proBMP-15 but remains associated with mature BMP-15 (8). Mature BMP-15 exists in 16 kDa and 17 kDa forms which are distinguishable by the presence of O-linked glycosylation on the 17 kDa form (8). Mature BMP-15 is phosphorylated, a modification which is required for the stimulation of GC proliferation (9). BMP-15 exerts its effects through interactions with BMPR‑IB/ALK6 and BMPR‑II (9-11). Mature BMP-15 forms 34 kDa noncovalently-linked homodimers and 37 kDa heterodimers with mature GDF-9 (12). Both of these oocyte-expressed factors lack the cysteine that mediates disulfide-linked dimerization in most TGF-beta superfamily ligands (1). Although heterodimerization with GDF-9 may limit the secretion of active BMP‑15, these two factors synergize in promoting oocyte survival and folliculogenesis (12, 13). Mature human BMP-15 shares 70%, 68%, and 78% aa sequence identity with mouse, rat, and sheep BMP-15, respectively. It shares 27%-38% aa sequence with other BMPs.

  1. Moore, R.K. and S. Shimasaki (2005) Mol. Cell. Endocrinol. 234:67.
  2. Otsuka, F. et al. (2000) J. Biol. Chem. 275:39523.
  3. Sugiura, K. et al. (2007) Development 134:2593.
  4. Su, Y.-Q. et al. (2008) Development 135:111.
  5. Otsuka, F. and S. Shimasaki (2002) Proc. Natl. Acad. Sci. 99:8060.
  6. Otsuka, F. et al. (2001) J. Biol. Chem. 276:11387.
  7. Dube, J.L. et al. (1998) Mol. Endocrinol. 12:1809.
  8. Saito, S. et al. (2008) Prot. Sci. 17:362.
  9. McMahon, H.E. et al. (2008) Endocrinology 149:812.
  10. Moore, R.K. et al. (2003) J. Biol. Chem. 278:304.
  11. Edwards, S.J. et al. (2008) Endocrinology 149:1026.
  12. Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
  13. Yan, C. et al. (2001) Mol. Endocrinol. 15:854.

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Publications for BMP-15/GDF-9B (5096-BM)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Goat.

We have publications tested in 4 applications: Bioassay, Ctrl, Surface Plasmon Resonance, WB Standard.


Filter By Application
Bioassay
(9)
Ctrl
(1)
Surface Plasmon Resonance
(1)
WB Standard
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
Bovine
(1)
Goat
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 5096-BM Applications Species
C Liu, B Yuan, H Chen, M Xu, X Sun, J Xu, Y Gao, C Chen, H Jiang, J Zhang Effects of MiR-375-BMPR2 as a Key Factor Downstream of BMP15/GDF9 on the Smad1/5/8 and Smad2/3 Signaling Pathways Cell. Physiol. Biochem., 2018;46(1):213-225. 2018 [PMID: 29587293] (Bioassay, Bovine) Bioassay Bovine
D Jung, J Xiong, M Ye, X Qin, L Li, S Cheng, M Luo, J Peng, J Dong, F Tang, W Shen, MM Matzuk, K Kee In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells Nat Commun, 2017;8(0):15680. 2017 [PMID: 28604658] (Bioassay, Human) Bioassay Human
T Jiajie, Y Yanzhou, AC Hoi-Hung, C Zi-Jiang, C Wai-Yee Conserved miR-10 family represses proliferation and induces apoptosis in ovarian granulosa cells Sci Rep, 2017;7(0):41304. 2017 [PMID: 28112253] (Bioassay, Mouse) Bioassay Mouse
N Kong, X Xu, Y Zhang, Y Wang, X Hao, Y Zhao, J Qiao, G Xia, M Zhang Natriuretic peptide type C induces sperm attraction for fertilization in mouse Sci Rep, 2017;7(0):39711. 2017 [PMID: 28054671] (Bioassay, Mouse) Bioassay Mouse
Mottershead D, Sugimura S, Al-Musawi S, Li J, Richani D, White M, Martin G, Trotta A, Ritter L, Shi J, Mueller T, Harrison C, Gilchrist R Cumulin, an Oocyte-secreted Heterodimer of the Transforming Growth Factor-beta Family, Is a Potent Activator of Granulosa Cells and Improves Oocyte Quality. J Biol Chem, 2015;290(39):24007-20. 2015 [PMID: 26254468] (WB Standard, Human) WB Standard Human
Sudiman J, Sutton-McDowall M, Ritter L, White M, Mottershead D, Thompson J, Gilchrist R Bone morphogenetic protein 15 in the pro-mature complex form enhances bovine oocyte developmental competence. PLoS ONE, 2014;9(7):e103563. 2014 [PMID: 25058588] (Bioassay, Human) Bioassay Human
Chang H, Cheng J, Klausen C, Leung P BMP15 suppresses progesterone production by down-regulating StAR via ALK3 in human granulosa cells. Mol Endocrinol, 2013;27(12):2093-104. 2013 [PMID: 24140593] (Bioassay, Human) Bioassay Human
Demars J, Fabre S, Sarry J, Rossetti R, Gilbert H, Persani L, Tosser-Klopp G, Mulsant P, Nowak Z, Drobik W, Martyniuk E, Bodin L Genome-wide association studies identify two novel BMP15 mutations responsible for an atypical hyperprolificacy phenotype in sheep. PLoS Genet, 2013;9(4):e1003482. 2013 [PMID: 23637641] (Ctrl, Human) Ctrl Human
Mottershead D, Ritter L, Gilchrist R Signalling pathways mediating specific synergistic interactions between GDF9 and BMP15. Mol Hum Reprod, 2012;18(3):121-8. 2012 [PMID: 21911477] (Bioassay, Mouse) Bioassay Mouse
Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011;286(34):30034-46. 2011 [PMID: 21737454] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol BMP15
Entrez
Uniprot