Myeloid leukemia factor 1 Antibody (OTI3A3)

Western Blot: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Myeloid leukemia factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Myeloid leukemia factor 1.

Immunocytochemistry/Immunofluorescence: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Myeloid leukemia factor 1.

Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Human tonsil using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.

Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - HEK293T cells transfected with either Myeloid leukemia factor 1 Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MLF1 antibody , and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Myeloid leukemia factor 1 Antibody (OTI3A3) [NBP2-02052] - Staining of paraffin-embedded Human pancreas tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI3A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Myeloid leukemia factor 1 Antibody (OTI3A3) Summary

• Description: Novus Biologicals Mouse Myeloid leukemia factor 1 Antibody (OTI3A3) (NBP2-02052) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human MLF1(NP_071888) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MLF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 30.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Myeloid leukemia factor 1 Antibody (OTI3A3)

• Myelodysplasia-myeloid leukemia factor 1
• myeloid leukemia factor 1

Background

This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MLF1
• Entrez:
  • 4291(Human)