MLF1 Interacting Protein [p Thr78] Antibody

Images

 
Western Blot: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Shows detection of MLF1IP phosphorylated at Thr78. HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or ...read more
Immunohistochemistry: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Used at 20 g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows ...read more
Immunohistochemistry: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Analysis of MLF1IP pT78 at the kinetochores of HeLa cells in different phases of the cell cycle. Fluorescent signals were detectable at ...read more

Product Details

Summary
Reactivity Hu, Bv, Ca, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MLF1 Interacting Protein [p Thr78] Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein. The immunogen peptide is phosphorylated at Thr78.
Modification
p Thr78
Specificity
This antibody is specific for human Mlf1 phospho T78 and surrounding amino acids.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MLF1IP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:5000-1:25000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 20 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Expect a band approximately 65 kDa in size corresponding - MLF1IP protein by western blotting in the appropriately stimulated tissue, cell lysate or extract.
Publications
Read Publication using NBP1-77938.

Reactivity Notes

Reactive in human, dog, bovine, chimpanzee

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MLF1 Interacting Protein [p Thr78] Antibody

  • CENP-50
  • CENP-U
  • CENPU50
  • CENPUMLF1-interacting protein
  • centromere protein of 50 kDa
  • centromere protein U
  • FLJ23468
  • ICEN24
  • Interphase centromere complex protein 24
  • KLIP1CENP50
  • KSHV latent nuclear antigen interacting protein 1
  • KSHV latent nuclear antigen-interacting protein 1
  • MLF1 interacting protein
  • PBIP1
  • Polo-box-interacting protein 1

Background

Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/ anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor,

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MLF1IP
Uniprot