MLF1 Interacting Protein Antibody

Images

 
Western Blot: MLF1 Interacting Protein Antibody [NB110-85541] - Analysis of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated ...read more
Immunohistochemistry: MLF1 Interacting Protein Antibody [NB110-85541] - Tissue: heart. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-MLF1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase ...read more
Immunohistochemistry: MLF1 Interacting Protein Antibody [NB110-85541] - Tissue: brain, cerebellum. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-MLF1 at 5 ug/mL for 1 h at RT. Secondary antibody: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.88 mg/ml

Order Details

MLF1 Interacting Protein Antibody Summary

Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a 200 residue recombinant protein corresponding to the amino terminal end of human MLF1IP protein.
Specificity
This is directed against human MLF1IP protein. A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, chimpanzee (98%), horse (72%), bovine (69%), dog (65%), mouse (56%), rat (55%) and chicken (47%) sources based on identity with the immunizing sequence. Reactivity against homologues from other sources is not known.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MLF1IP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:1400000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Expect a band approximately 65 kDa in size corresponding - MLF1IP protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reacts with Human. A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, chimpanzee (98%), horse (72%), bovine (69%), dog (65%), mouse (56%), rat (55%) and chicken (47%) sources based on homology with the immunizing sequence.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
0.88 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MLF1 Interacting Protein Antibody

  • CENP-50
  • CENP-U
  • CENPU50
  • CENPUMLF1-interacting protein
  • centromere protein of 50 kDa
  • centromere protein U
  • FLJ23468
  • ICEN24
  • Interphase centromere complex protein 24
  • KLIP1CENP50
  • KSHV latent nuclear antigen interacting protein 1
  • KSHV latent nuclear antigen-interacting protein 1
  • MLF1 interacting protein
  • PBIP1
  • Polo-box-interacting protein 1

Background

Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MLF1IP
Entrez