Mkl1 Antibody - BSA Free

Western Blot: Mkl1 Antibody [NBP3-04626] - Analysis of extracts of various cell lines, using Mkl1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Immunocytochemistry/ Immunofluorescence: Mkl1 Antibody - BSA Free [Mkl1] - Immunofluorescence analysis of U-2 OS cells using Mkl1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry: Mkl1 Antibody - BSA Free [Mkl1] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Mkl1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry: Mkl1 Antibody - BSA Free [Mkl1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Mkl1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Mkl1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Mkl1 Antibody - BSA Free (NBP3-04626) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-Mkl1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MKL1 (NP_065882.1). MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERSELVRMHILEETSAEPSLQAKQLKLKRARLADDLNEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVPSPLEARVSEPLLSATSASPTQVVSQLPMGRDSREMLFLAEQPPLPPPPLLPPSLTNGTTIPTAKSTPTLIKQSQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDRGAPPMDSSYAKILQQQQLFLQLQILNQQQQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRTFA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:20-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 98 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Mkl1 Antibody - BSA Free

• AMKL
• KIAA1438
• MALSAP and coiled-coil domain
• megakaryoblastic leukemia (translocation) 1
• Megakaryoblastic leukemia 1 protein
• Megakaryocytic acute leukemia protein
• MKL/myocardin-like protein 1
• Myocardin-related transcription factor A
• RNA-binding motif protein 15/megakaryoblastic leukemia-1 fusion protein

Background

MKL1 (megakaryoblastic leukemia 1 protein) was identified as part of a t(1;22) translocation with RBM15 associated with acute megakaryoblastic leukemia. It is a transcriptional coactivator of serum response factor (SRF) that promotes muscle differentiation and also plays a role in promoting maturation of megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mkl1 Antibody (NBP3-04626)(1)

Publication using NBP3-04626

• Ding Q, Wang GJ, Xue LF et al. p38MAPK silencing attenuates scar proliferation after cleft palate repair surgery in rats via MRTF-A/SRF pathway Experimental cell research Aug 15 2022 [PMID: 35690133] (IHC-P, Rat)

Bioinformatics

• Gene Symbol: MRTFA