Myocardin Antibody (355521)

Western blot shows lysates of MCF‑7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 355521
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Myocardin Antibody (355521) Summary

• Immunogen: E. coli-derived recombinant human Myocardin
  Met97-Leu290
  Accession # Q8IZQ8
• Specificity: Detects human and mouse Myocardin in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYOCD
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myocardin Antibody (355521)

• BSAC2A
• MYCD
• Myocardin
• MYOCD
• Srfcp

Background

Myocardin (MYOCD) is a transcriptional co-activator necessary for differentiation of smooth muscle cells. MYOCD functions by binding the transcription factor Serum Response Factor (SRF) and stimulating smooth muscle cell-specific gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myocardin Antibody (MAB4028)(10)

Publications using MAB4028

• J Dai, Q Zhou, J Chen, ML Rexius-Hal, J Rehman, G Zhou Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway Nat Commun, 2018-09-21;9(1):3850. 2018-09-21 [PMID: 30242159] (IHC, Western Blot, Human)
• Gang Xi Downregulation of Insulin Receptor Substrate-1 During Hyperglycemia Induces Vascular Smooth Muscle Cell Dedifferentiation J. Biol. Chem, 2016-12-21;0(0):. 2016-12-21 [PMID: 28003360] (Western Blot, Mouse)
• Tianji Chen, Qiyuan Zhou, Haiyang Tang, Melike Bozkanat, Jason X-J Yuan, J Usha Raj, Guofei Zhou miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation. Journal of the American Heart Association, 2016-12-05;0(0):2047-9980. 2016-12-05 [PMID: 27919930] (Western Blot, Human)
• Madonna R, Geng Y, Bolli R, Rokosh G, Ferdinandy P, Patterson C, De Caterina R Co-activation of nuclear factor-kappaB and myocardin/serum response factor conveys the hypertrophy signal of high insulin levels in cardiac myoblasts. J Biol Chem, 2014-05-22;289(28):19585-98. 2014-05-22 [PMID: 24855642] (Western Blot, Canine, Mouse)
• Yao Y, Chen Z, Norris E, Strickland S Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. Nat Commun, 2014-03-03;5(0):3413. 2014-03-03 [PMID: 24583950] (Western Blot, Mouse)
• Abdalla M, Goc A, Segar L, Somanath P Akt1 mediates alpha-smooth muscle actin expression and myofibroblast differentiation via myocardin and serum response factor. J Biol Chem, 2013-10-08;288(46):33483-93. 2013-10-08 [PMID: 24106278] (Western Blot, Mouse)
• Madonna R, Taylor D, Geng Y, De Caterina R, Shelat H, Perin E, Willerson J Transplantation of mesenchymal cells rejuvenated by the overexpression of telomerase and myocardin promotes revascularization and tissue repair in a murine model of hindlimb ischemia. Circ Res, 2013-06-18;113(7):902-14. 2013-06-18 [PMID: 23780385] (Western Blot, Mouse)
• Lu , Tzong-Sh, Lim , Kenneth, Molostvov , Guerman, Yang , Yun-Chun, Yiao , Szu-Yu, Zehnder , Daniel, Hsiao , Li-Li Induction of intracellular heat-shock protein 72 prevents the development of vascular smooth muscle cell calcification. Cardiovasc Res, 2012-08-29;96(3):524-32. 2012-08-29 [PMID: 22933322] (Western Blot, Human)
• Pfisterer , Larissa, Feldner , Anja, Hecker , Markus, Korff , Thomas Hypertension impairs myocardin function: a novel mechanism facilitating arterial remodelling. Cardiovasc Res, 2012-07-26;96(1):120-9. 2012-07-26 [PMID: 22843699] (IHC-P, Mouse)
• Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A Down-regulation of Kruppel-like Factor-4 (KLF4) by MicroRNA-143/145 Is Critical for Modulation of Vascular Smooth Muscle Cell Phenotype by Transforming Growth Factor-{beta} and Bone Morphogenetic Protein 4. J. Biol. Chem., 2011-06-13;286(32):28097-110. 2011-06-13 [PMID: 21673106] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MYOCD
• Uniprot:
  • Q8IZQ8()