Myocardin Antibody (355521)

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Western blot shows lysates of MCF‑7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
355521
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Myocardin Antibody (355521) Summary

Immunogen
E. coli-derived recombinant human Myocardin
Met97-Leu290
Accession # Q8IZQ8
Specificity
Detects human and mouse Myocardin in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MYOCD
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB4028 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myocardin Antibody (355521)

  • BSAC2A
  • MYCD
  • Myocardin
  • MYOCD
  • Srfcp

Background

Myocardin (MYOCD) is a transcriptional co-activator necessary for differentiation of smooth muscle cells. MYOCD functions by binding the transcription factor Serum Response Factor (SRF) and stimulating smooth muscle cell-specific gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu
Applications: WB

Publications for Myocardin Antibody (MAB4028)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 2 applications: IHC-P, Western Blot.


Filter By Application
IHC-P
(1)
Western Blot
(10)
All Applications
Filter By Species
Human
(5)
Mouse
(5)
Canine
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB4028 Applications Species
J Dai, Q Zhou, J Chen, ML Rexius-Hal, J Rehman, G Zhou Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway Nat Commun, 2018;9(1):3850. 2018 [PMID: 30242159] (Western Blot, Human) Western Blot Human
Gang Xi Downregulation of Insulin Receptor Substrate-1 During Hyperglycemia Induces Vascular Smooth Muscle Cell Dedifferentiation J. Biol. Chem, 2016;0(0):. 2016 [PMID: 28003360] (Western Blot, Mouse) Western Blot Mouse
miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation J Am Heart Assoc, 2016;5(12):. 2016 [PMID: 27919930] (Western Blot, Human) Western Blot Human
Tianji Chen, Qiyuan Zhou, Haiyang Tang, Melike Bozkanat, Jason X-J Yuan, J Usha Raj, Guofei Zhou miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation. Journal of the American Heart Association, 2016;0(0):2047-9980. 2016 [PMID: 27919930] (Western Blot, Human) Western Blot Human
Madonna R, Geng Y, Bolli R, Rokosh G, Ferdinandy P, Patterson C, De Caterina R Co-activation of nuclear factor-kappaB and myocardin/serum response factor conveys the hypertrophy signal of high insulin levels in cardiac myoblasts. J Biol Chem, 2014;289(28):19585-98. 2014 [PMID: 24855642] (Western Blot, Canine) Western Blot Canine
Yao Y, Chen Z, Norris E, Strickland S Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. Nat Commun, 2014;5(0):3413. 2014 [PMID: 24583950] (Western Blot, Mouse) Western Blot Mouse
Abdalla M, Goc A, Segar L, Somanath P Akt1 mediates alpha-smooth muscle actin expression and myofibroblast differentiation via myocardin and serum response factor. J Biol Chem, 2013;288(46):33483-93. 2013 [PMID: 24106278] (Western Blot, Mouse) Western Blot Mouse
Madonna R, Taylor D, Geng Y, De Caterina R, Shelat H, Perin E, Willerson J Transplantation of mesenchymal cells rejuvenated by the overexpression of telomerase and myocardin promotes revascularization and tissue repair in a murine model of hindlimb ischemia. Circ Res, 2013;113(7):902-14. 2013 [PMID: 23780385] (Western Blot, Mouse) Western Blot Mouse
Lu, Tzong-Sh, Lim, Kenneth, Molostvov, Guerman, Yang, Yun-Chun, Yiao, Szu-Yu, Zehnder, Daniel, Hsiao, Li-Li Induction of intracellular heat-shock protein 72 prevents the development of vascular smooth muscle cell calcification. Cardiovasc Res, 2012;96(3):524-32. 2012 [PMID: 22933322] (Western Blot, Human) Western Blot Human
Pfisterer, Larissa, Feldner, Anja, Hecker, Markus, Korff, Thomas Hypertension impairs myocardin function: a novel mechanism facilitating arterial remodelling. Cardiovasc Res, 2011;96(1):120-9. 2011 [PMID: 22843699] (IHC-P, Mouse) IHC-P Mouse
Show All 11 Publications.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MYOCD
Uniprot