Home » MKL2 » MKL2 Antibodies » MKL2 Antibody (NBP1-46209)

MKL2 Antibody (NBP1-46209)

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: MKL2 Antibody [NBP1-46209] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MKL2 antibody NBP1-46209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL2 was also immunoprecipitated by rabbit anti-MKL2 antibody NBP1-46208, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Western Blot: MKL2 Antibody [NBP1-46209] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity ...read more
Immunohistochemistry: MKL2 Antibody [NBP1-46209] - Sample: FFPE section of human prostate carcinoma and linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-MKL2 used at a dilution of 1:200 (1ug/ml) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IB, ICC/IF, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

  

Biological Strategies

      

Order Details

100% Guarantee on every product

MKL2 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1038 and 1088 of human Megakaryoblastic Leukemia 2 using the numbering given in entry NP_054767.3 (GeneID 57496).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MKL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoblotting 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope Retrieval with Citrate Buffer is recommended.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-46209 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MKL2 Antibody

  • DKFZp686J1745
  • FLJ31823
  • FLJ45623
  • KIAA1243
  • megakaryoblastic leukemia 2
  • MKL/myocardin-like 2
  • MKL/myocardin-like protein 2
  • MRTFB
  • MRTF-B
  • myocardin-related transcription factor B
  • NPD001

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Mkl1 Antibody
NBP1-88498
Western Blot: Mkl1 Antibody [NBP1-88498] - Analysis in human cell line HL-60.
Immunocytochemistry/Immunofluorescence: Mkl1 Antibody [NBP1-88498] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lymph node shows cytoplasmic positivity in lymphocytes.
(5 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Serum Response
Factor SRF Antibody
NBP1-87814
Western Blot: Serum Response Factor SRF Antibody [NBP1-87814] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: MOLT-4
Immunocytochemistry/Immunofluorescence: Serum Response Factor SRF Antibody [NBP1-87814] - Staining of human cell line U-251MG shows positivity in nucleus but not nucleoli and endoplasmic reticulum.
Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody [NBP1-87814] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Myocardin Antibody
(355521)
MAB4028
Western blot shows lysates of MCF‑7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(8 Publications)
Species: Hu, Mu
Applications: WB
RhoA Antibody
NB100-91273
Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.
Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.
(1 Review)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
TIRAP (TLR2 and
TLR4) Inhibitor Pepti ...
NBP2-26245
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - The TLR4 reporter cell line responds to LPS. The TLR2 / TLR4 inhibitor specifically inhibits TLR4 activation upon LPS stimulation in a dose-response manner.
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 /4 Inhibitor suppressed the Pam3CSK4-induced TLR2/TLR1 activity [A] and the LPS-induced TLR4 activity [B] in a dose-response manner, of which IC50 values were measured as 17.2 uM and 10.47 uM, respectively.
(4 Publications)
Species: Hu, Mu
MAL Antibody
NBP2-22522
Western Blot: MAL Antibody [NBP2-22522] - Analysis of 50ug of mouse mpkCCD and Xenopus 2f3 whole cell lysates per well.
Species: Hu, Mu, Rt, Xp
Applications: WB
CD8 alpha Antibody
(53-6.7)
NBP1-49045
Immunocytochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/ml and incubated for 3 hours at room temperature.
Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
(1 Review)
(8 Publications)
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
Rhodopsin Antibody
(1D4)
NBP1-47602
Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (1D4) [NBP1-47602] - analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.
(1 Publication)
Species: Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
PCTAIRE1 Antibody
NBP1-92248
Western Blot: PCTAIRE1 Antibody [NBP1-92248] - Analysis in human cell line HEK 293.
Immunohistochemistry-Paraffin: PCTAIRE1 Antibody [NBP1-92248] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells, with additional strong membranous positivity.
Western Blot: PCTAIRE1 Antibody [NBP1-92248] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
RhoD Antibody (2F7)
NBP2-01462
Western Blot: RhoD Antibody (2F7) [NBP2-01462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RhoD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RhoD.
Immunocytochemistry/Immunofluorescence: RhoD Antibody (2F7) [NBP2-01462] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RhoD.
Flow Cytometry: RhoD Antibody (2F7) [NBP2-01462] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RhoD antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: WB, Flow, ICC/IF
HNF-4 alpha/NR2A1
Antibody (843716) [Un ...
MAB4605
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 10 µg of cytoplasmic (Cyto) and 5 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HNF‑4 alpha /NR2A1 Monoclonal Antibody (Catalog # MAB4605) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for HNF‑4 alpha /NR2A1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
BG01V human embryonic stem cell line differentiated to endodermal cells (StemXVivo Endoderm Kit, Catalog # SC019B) was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.                               
(1 Review)
Species: Hu
Applications: WB, Simple Western, ICC, ICFlow
Cdk5 Antibody (4E4)
NBP2-37602
Western Blot: Cdk5 Antibody (4E4) [NBP2-37602] - Western blot analysis using CDK5 mouse mAb against Hela (1), K562 (2), PC-12 (3) and Cos7 (4) cell lysate.
Immunocytochemistry/Immunofluorescence: Cdk5 Antibody (4E4) [NBP2-37602] - Immunofluorescence analysis of GC7901 cells using CDK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cdk5 Antibody (4E4) [NBP2-37602] - Analysis of paraffin-embedded cerebellum tissues (left) and brain tissues (right) using CDK5 mouse mAb with DAB staining.
Species: Hu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
DIAPH3 Antibody
(4D5)
H00081624-M01
Western Blot: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 monoclonal antibody (M01), clone 4D5.Lane 1: DIAPH3 transfected lysate (Predicted MW: 98.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of monoclonal antibody to DIAPH3 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: DIAPH3 Antibody (4D5) [H00081624-M01] - Detection limit for recombinant GST tagged DIAPH3 is 1 ng/ml as a capture antibody.
Species: Hu
Applications: WB, ELISA, ICC/IF
c-Fos Antibody
(2H2)
NBP2-50037
Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. NBP2-50037 recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel: Blot was stripped and probed with monoclonal antibody against GAPDH (NB300-221) used as loading control.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).
(1 Review)
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
ABRA Antibody
NBP1-80716
Immunohistochemistry-Paraffin: ABRA Antibody [NBP1-80716] - Staining of human skeletal muscle showed strong cytoplasmic positivity.
Species: Hu
Applications: IHC, IHC-P
Exosome component
1 Antibody (1H9)
NBP2-45545
Western Blot: Exosome component 1 Antibody (1H9) [NBP2-45545] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Exosome component 1.
Immunohistochemistry: Exosome component 1 Antibody (1H9) [NBP2-45545] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Exosome component 1 Antibody (1H9) [NBP2-45545] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu
Applications: WB, IHC
EGR2 Antibody
NB110-59723
Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.
Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - EGR2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunofluorescence: EGR2 Antibody [NB110-59723] - EGR2 staining on E13.5 dorsal root ganglion and neural tube.
(1 Review)
Species: Hu, Mu, Po
Applications: WB, ICC/IF, IF
C11orf95 Antibody
NBP2-55275
Immunocytochemistry/Immunofluorescence: C11orf95 Antibody [NBP2-55275] - Staining of human cell line U-251 MG shows localization to intermediate filaments.
Species: Hu
Applications: ICC/IF

Publications for MKL2 Antibody (NBP1-46209)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IB, ICC/IF.


Filter By Application
IB
(1)
ICC/IF
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for MKL2 Antibody (NBP1-46209) (0)

There are no reviews for MKL2 Antibody (NBP1-46209). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MKL2 Antibody (NBP1-46209) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MKL2 Products

Related Products by Gene

ABRA, C11ORF95, CD8A, CDK16, CDK5, CHROMOSOME 11 OPEN READING FRAME 95, DIAPH3, EGR2, EXOSC1, FOS, HNF4A, MAL, MKL1, MYOCD, RHO, RHOA, RHOD, SMOOTH MUSCLE ALPHA-ACTIN, SRF, TIRAP

Bioinformatics Tool for MKL2 Antibody (NBP1-46209)

Discover related pathways, diseases and genes to MKL2 Antibody (NBP1-46209). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MKL2 Antibody (NBP1-46209)

Discover more about diseases related to MKL2 Antibody (NBP1-46209).
 

Pathways for MKL2 Antibody (NBP1-46209)

View related products by pathway.

PTMs for MKL2 Antibody (NBP1-46209)

Learn more about PTMs related to MKL2 Antibody (NBP1-46209).

Blogs on MKL2

There are no specific blogs for MKL2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MKL2 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol MKL2
Entrez
Uniprot