MKL2 Antibody

Western Blot: MKL2 Antibody [NBP1-46209] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MKL2 antibody NBP1-46209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL2 was also immunoprecipitated by rabbit anti-MKL2 antibody NBP1-46208, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Immunohistochemistry: MKL2 Antibody [NBP1-46209] - Sample: FFPE section of human prostate carcinoma and linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-MKL2 used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MKL2 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 1038 and 1088 of human Megakaryoblastic Leukemia 2 using the numbering given in entry NP_054767.3 (GeneID 57496).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MKL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
  • Immunoblotting 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope Retrieval with Citrate Buffer is recommended.
• Theoretical MW: 118 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MKL2 Antibody

• DKFZp686J1745
• FLJ31823
• FLJ45623
• KIAA1243
• megakaryoblastic leukemia 2
• MKL/myocardin-like 2
• MKL/myocardin-like protein 2
• MRTFB
• MRTF-B
• myocardin-related transcription factor B
• NPD001

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MKL2 Antibody (NBP1-46209)(1)

Publication using NBP1-46209

• Seifert A, Posern G. Tightly controlled MRTF-A activity regulates epithelial differentiation during formation of mammary acini. Breast Cancer Res. 2017 Jun 07 [PMID: 28592291] (ICC/IF, IB, Human)

Bioinformatics

• Gene Symbol: MKL2
• Entrez:
  • 57496(Human)
  • 239719(Mouse)
• Uniprot:
  • Q9ULH7(Human)