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MKL2 Antibody

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Western Blot: MKL2 Antibody [NBP1-46209] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MKL2 antibody NBP1-46209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL2 was also immunoprecipitated by rabbit anti-MKL2 antibody NBP1-46208, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Western Blot: MKL2 Antibody [NBP1-46209] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity ...read more
Immunohistochemistry: MKL2 Antibody [NBP1-46209] - Sample: FFPE section of human prostate carcinoma and linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-MKL2 used at a dilution of 1:200 (1ug/ml) ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Ca, ChHa, Eq, GP, Mk, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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MKL2 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1038 and 1088 of human Megakaryoblastic Leukemia 2 using the numbering given in entry NP_054767.3 (GeneID 57496).
Predicted Species
Equine (100%), Primate (100%), Bovine (100%), Guinea Pig (100%), Canine (100%), Monkey (100%), Rabbit (100%), Chinese Hamster (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
MKL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Rhesus Monkey, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque, Naked mole rat, Small-eared galago and Northern white-cheeked gibbon.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MKL2 Antibody

  • DKFZp686J1745
  • FLJ31823
  • FLJ45623
  • KIAA1243
  • megakaryoblastic leukemia 2
  • MKL/myocardin-like 2
  • MKL/myocardin-like protein 2
  • MRTFB
  • MRTF-B
  • myocardin-related transcription factor B
  • NPD001

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Mkl1 Antibody [NBP1-88498] - Analysis in human cell line HL-60.
Immunocytochemistry/Immunofluorescence: Mkl1 Antibody [NBP1-88498] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lymph node shows cytoplasmic positivity in lymphocytes.
(5 Publications)
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Western Blot: Serum Response Factor SRF Antibody [NBP1-87814] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: MOLT-4
Immunocytochemistry/Immunofluorescence: Serum Response Factor SRF Antibody [NBP1-87814] - Staining of human cell line U-251MG shows positivity in nucleus but not nucleoli and endoplasmic reticulum.
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Western blot shows lysates of MCF‑7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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RhoA Antibody
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Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.
Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.
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TIRAP (TLR2 and
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NBP2-26245
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - The TLR4 reporter cell line responds to LPS. The TLR2 / TLR4 inhibitor specifically inhibits TLR4 activation upon LPS stimulation in a dose-response manner.
TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 /4 Inhibitor suppressed the Pam3CSK4-induced TLR2/TLR1 activity [A] and the LPS-induced TLR4 activity [B] in a dose-response manner, of which IC50 values were measured as 17.2 uM and 10.47 uM, respectively.
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Western Blot: MAL Antibody [NBP2-22522] - Analysis of 50ug of mouse mpkCCD and Xenopus 2f3 whole cell lysates per well.
Species: Hu, Mu, Rt, Xp
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Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
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Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important to avoid boiling samples containing this protein in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the Rhodopsin protein and appearance of more of this high molecular weight material.
Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (B630) [NBP2-25160] - High magnification confocal image of pig retinal section stained with NBP2-25160 (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).
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NBP1-92248
Western Blot: PCTAIRE1 Antibody [NBP1-92248] - Analysis in human cell line HEK 293.
Immunohistochemistry-Paraffin: PCTAIRE1 Antibody [NBP1-92248] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells, with additional strong membranous positivity.
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NBP2-01462
Western Blot: RhoD Antibody (2F7) [NBP2-01462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RhoD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RhoD.
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Flow Cytometry: RhoD Antibody (2F7) [NBP2-01462] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RhoD antibody, and then analyzed by flow cytometry.
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HNF-4 alpha/NR2A1
Antibody (843716) [Un ...
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Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for HNF‑4 alpha /NR2A1 at approximately 62 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human HNF‑4 alpha /NR2A1 Monoclonal Antibody (Catalog # MAB4605). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
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Western Blot: Cdk5 Antibody (4E4) [NBP2-37602] - Western blot analysis using CDK5 mouse mAb against Hela (1), K562 (2), PC-12 (3) and Cos7 (4) cell lysate.
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Western Blot: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 monoclonal antibody (M01), clone 4D5.Lane 1: DIAPH3 transfected lysate (Predicted MW: 98.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of monoclonal antibody to DIAPH3 on HeLa cell. Antibody concentration 10 ug/ml.
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c-Fos Antibody
(2H2)
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Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. NBP2-50037 recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel: Blot was stripped and probed with monoclonal antibody against GAPDH (NB300-221) used as loading control.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).
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Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
ABRA Antibody
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Western Blot: ABRA Antibody [NBP1-80716] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-12
Immunohistochemistry-Paraffin: ABRA Antibody [NBP1-80716] - Staining of human skeletal muscle showed strong cytoplasmic positivity.
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Applications: WB, IHC, IHC-P
Exosome component
1 Antibody (1H9)
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Western Blot: Exosome component 1 Antibody (1H9) [NBP2-45545] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Exosome component 1.
Immunohistochemistry: Exosome component 1 Antibody (1H9) [NBP2-45545] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Exosome component 1 Antibody (1H9) [NBP2-45545] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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EGR2 Antibody
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Western Blot: EGR2 Antibody [NB110-59723] - Detection of EGR2 in human fetal lung tissue using NB110-59723.
Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB110-59723] - EGR2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: EGR2 Antibody [NB110-59723] - EGR2 staining on E13.5 dorsal root ganglion and neural tube.
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C11orf95 Antibody
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Immunocytochemistry/Immunofluorescence: C11orf95 Antibody [NBP2-55275] - Staining of human cell line U-251 MG shows localization to intermediate filaments.
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Bioinformatics

Gene Symbol MKL2
Entrez
Uniprot

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