DNER Antibody [Unconjugated]

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DNER was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Goat Anti-Mouse DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2254) at 5 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

DNER Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DNER
Ala26-His637
Accession # Q8JZM4
Specificity
Detects mouse DNER in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human DNER is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DNER
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2254 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DNER Antibody [Unconjugated]

  • bet
  • delta and Notch-like epidermal growth factor-related receptor
  • delta/notch-like EGF repeat containing
  • delta-notch-like EGF repeat-containing transmembrane
  • DNER
  • H_NH0150O02.1
  • UNQ26
  • WUGSC:H_NH0150O02.1

Background

DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNER Antibody (AF2254)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(2)
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Human
(2)
Mouse
(3)
Chicken
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF2254 Applications Species
YT Shue, AP Drainas, NY Li, SM Pearsall, D Morgan, N Sinnott-Ar, SQ Hipkins, GL Coles, JS Lim, AE Oro, KL Simpson, C Dive, J Sage A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs Nature Communications, 2022;13(1):2690. 2022 [PMID: 35577801] (IHC, Mouse) IHC Mouse
M Marino, L Zhou, MY Rincon, Z Callaerts-, J Verhaert, J Wahis, E Creemers, L Yshii, K Wierda, T Saito, C Marneffe, I Voytyuk, Y Wouters, M Dewilde, SI Duqué, C Vincke, Y Levites, TE Golde, TC Saido, S Muylderman, A Liston, B De Stroope, MG Holt AAV-mediated delivery of an anti-BACE1 VHH alleviates pathology in an Alzheimer's disease model Embo Molecular Medicine, 2022;14(4):e09824. 2022 [PMID: 35352880] (Western Blot, Human) Western Blot Human
C Ballester-, TM Conlon, Z Ertüz, FR Greiffo, M Irmler, SE Verleden, J Beckers, IE Fernandez, O Eickelberg, AÖ Yildirim The Notch ligand DNER regulates macrophage IFN? release in chronic obstructive pulmonary disease EBioMedicine, 2019;0(0):. 2019 [PMID: 31060902] (IHC-P, Mouse) IHC-P Mouse
J Du, X Wang, X Zhang, X Zhang, H Jiang DNER modulates the length, polarity and synaptogenesis of spiral ganglion neurons via the Notch signaling pathway Mol Med Rep, 2018;17(2):2357-2365. 2018 [PMID: 29207144] (Western Blot, Mouse) Western Blot Mouse
Delta/Notch-Like EGF-Related Receptor (DNER) Is Not a Notch Ligand PLoS ONE, 2016;11(9):e0161157. 2016 [PMID: 27622512] (ICC, Human) ICC Human
Kowalik L, Hudspeth AJ A search for factors specifying tonotopy implicates DNER in hair-cell development in the chick's cochlea. Dev. Biol., 2011;354(2):221-31. 2011 [PMID: 21497156] (IHC-Fr, Chicken) IHC-Fr Chicken

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Bioinformatics

Gene Symbol DNER
Uniprot