Mineralocorticoid R/NR3C2 Antibody - Azide and BSA Free

Western Blot: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:107 kDaObserved band size:107 kDa(We are unsure as to the identity of these extra bands.)

Immunocytochemistry/Immunofluorescence: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] - Staining of NR3C2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NR3C2 polyclonal antibody (1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.

Immunoprecipitation: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate4 uL anti-NR3C2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NR3C2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 107 kDaObserved band size: 107 kDa

Simple Western: Mineralocorticoid R/NR3C2 Antibody [NBP2-99010] - Simple Western lane view shows lysates of A172. A specific band was detected for Mineralocorticoid R/NR3C2 at approximately 107 kDa (as indicated) using 20 ug/mL of Mineralocorticoid R/NR3C2.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Mineralocorticoid R/NR3C2 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Mineralocorticoid R/NR3C2.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR3C2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Simple Western
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Mineralocorticoid R/NR3C2 Antibody - Azide and BSA Free

• FLJ41052
• MGC133092
• Mineralocorticoid R
• mineralocorticoid receptor 1
• mineralocorticoid receptor delta
• MineralocorticoidR
• MLR
• MLRmineralocorticoid receptor
• MRMCRaldosterone receptor
• NR3C2
• NR3C2VIT
• Nuclear receptor subfamily 3 group C member 2
• nuclear receptor subfamily 3, group C, member 2

Background

Steroid receptors are ligand dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain Mineralocorticoid Receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR3C2