MIF Antibody


Western Blot: MIF Antibody [NBP1-32192] - Sample (30 ug of whole cell lysate)A: Molt-4 15% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MIF Antibody [NBP1-32192] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.
Western Blot: MIF Antibody [NBP1-32192] - Sample (30 ug of whole cell lysate) NIH-3T3 15% SDS PAGE; antibody diluted at 1:1000.
ELISA: MIF Antibody [NBP1-32192] - ELISA detection of MIF [N1C3] capture at a concentration of 5 ug/mL and MIF C-term antibody for detection at a concentration of 1.5 ug/mL.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
1.0 mg/ml

Order Details

MIF Antibody Summary

Recombinant fragment corresponding to a region within amino acids 1 and 115 of MIF (Uniprot ID#P14174)
Secreted, Cytoplasm
Predicted Species
Rat (90%), Porcine (94%), Primate (100%), Sheep (91%), Bovine (91%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7.0).
0.01% Thimerosal
1.0 mg/ml
Immunogen affinity purified


Theoretical MW
12 kDa

Reactivity Notes

Rhesus Monkey (100%)

Alternate Names for MIF Antibody

  • EC
  • EC
  • GIFmacrophage migration inhibitory factor
  • GLIF
  • Glycosylation-inhibiting factor
  • L-dopachrome isomerase
  • L-dopachrome tautomerase
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • MMIF
  • Phenylpyruvate tautomerase


Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol MIF

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