Midkine Antibody [Unconjugated]

Images

 
Midkine was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑258‑PB) at 10 µg/mL overnight at 4 °C. ...read more
Midkine was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-258-PB) at 10 µg/mL overnight at 4 ...read more
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-258-PB) ...read more
Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Midkine at approximately 26 kDa (as indicated) using 100 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Midkine Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Midkine
Lys23-Asp143
Accession # P21741
Specificity
Detects human Midkine in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Midkine is observed, and less than 1% cross-reactivity with recombinant human Pleiotrophin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MDK
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 100 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
AF-258-PB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Midkine Antibody [Unconjugated]

  • Amphiregulin-associated protein
  • ARAP
  • MDK
  • MEK
  • Midgestation and kidney protein
  • midkine (neurite growth-promoting factor 2)
  • Midkine
  • MK1
  • MKARAP
  • NEGF2
  • NEGF2FLJ27379
  • Neurite outgrowth-promoting factor 2
  • Neurite outgrowth-promoting protein

Background

Midkine (MK) is a 15 kDa heparin-binding molecule originally cloned during a search for genes preferentially transcribed during retinoic acid (RA)-induced differentiation. Midkine belongs to a family of neurotrophic and developmentally-regulated heparin-binding molecules consisting of midkine, pleiotrophin (PTN/HBNF/OSF-1/HNGF-8) and the avian midkine homolog, RI-HB (for retinoic acid-inducible heparin-binding protein).

Midkine is a highly basic, nonglycosylated polypeptide that contains five intrachain disulfide bonds. The predicted molecular weight is approximately 13.3 kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Across species, MK shows 87% identity between the human and murine proteins. Between family members, human MK is approximately 50% identical to human PTN, with conservation of all 10 cysteines. Initial structure-function studies indicate that the C-terminal half of MK contains the principal heparin-binding site plus the molecule’s antigenicity and neurite-promoting sequences; while both the C- and N-termini are necessary for the molecule’s neurotrophic effects. Cells known to produce MK include endothelial cells, fetal astrocytes, renal proximal tubule epithelial cells and Wilms’ (kidney) tumor cells. MK has also been identified in the senile plaques of patients with Alzheimer’s disease. The pattern of expression of midkine during development strongly suggests a role for this factor both in epithelial-mesenchymal interactions and in development of the nervous system.

  1. Bohlen, P. and I. Kovesdi (1991) Prog. Growth Factor Res. 3:143.
  2. Muramatsu, T. (1993) Int. J. Dev. Biol. 37:183.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF-252-PB
Species: Hu
Applications: IHC, WB
288-TPN/CF
Species: Hu
Applications: BA
203-IL/CF
Species: Hu
Applications: BA
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
D6050
Species: Hu
Applications: ELISA
255-SC/CF
Species: Hu
Applications: BA
NBP2-27544
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
MAB7616
Species: Hu
Applications: CyTOF-ready, Flow, WB
NBP1-30027
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
233-FB/CF
Species: Hu
Applications: BA
DVE00
Species: Hu
Applications: ELISA
MEP00B
Species: Mu
Applications: ELISA
236-EG
Species: Hu
Applications: BA
AF8516
Species: Hu
Applications: ICC, IP, Simple Western, WB
AF1513
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
7268-CT
Species: Hu
Applications: BA
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
AF4090
Species: Hu
Applications: IHC, IP, Neut, WB

Publications for Midkine Antibody (AF-258-PB)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, Neutralization, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF-258-PB Applications Species
DH Shin, JY Jo, SH Kim, M Choi, C Han, BK Choi, SS Kim Midkine Is a Potential Therapeutic Target of Tumorigenesis, Angiogenesis, and Metastasis in Non-Small Cell Lung Cancer Cancers (Basel), 2020;12(9):. 2020 [PMID: 32847073] (IHC, Mouse) IHC Mouse
Marchionini DM, Lehrmann E, Chu Y, He B, Sortwell CE, Becker KG, Freed WJ, Kordower JH, Collier TJ Role of heparin binding growth factors in nigrostriatal dopamine system development and Parkinson's disease. Brain Res., 2007;1147(0):77-88. 2007 [PMID: 17368428] (Western Blot, Rat) Western Blot Rat
Li F, Shetty AK, Sugahara K Neuritogenic activity of chondroitin/dermatan sulfate hybrid chains of embryonic pig brain and their mimicry from shark liver. Involvement of the pleiotrophin and hepatocyte growth factor signaling pathways. J. Biol. Chem., 2007;282(5):2956-66. 2007 [PMID: 17145750] (Neutralization, Mouse) Neutralization Mouse
Kang HC, Kim IJ, Park HW, Jang SG, Ahn SA, Yoon SN, Chang HJ, Yoo BC, Park JG Regulation of MDK expression in human cancer cells modulates sensitivities to various anticancer drugs: MDK overexpression confers to a multi-drug resistance. Cancer Lett., 2007;247(1):40-7. 2007 [PMID: 16644108] (Western Blot, Human) Western Blot Human
Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptor. J. Biol. Chem., 2002;277(40):37492-502. 2002 [PMID: 12147681] (ICC, Human) ICC Human

Reviews for Midkine Antibody (AF-258-PB) (0)

There are no reviews for Midkine Antibody (AF-258-PB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Midkine Antibody (AF-258-PB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Midkine Products

Bioinformatics Tool for Midkine Antibody (AF-258-PB)

Discover related pathways, diseases and genes to Midkine Antibody (AF-258-PB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Midkine Antibody (AF-258-PB)

Discover more about diseases related to Midkine Antibody (AF-258-PB).
 

Pathways for Midkine Antibody (AF-258-PB)

View related products by pathway.

PTMs for Midkine Antibody (AF-258-PB)

Learn more about PTMs related to Midkine Antibody (AF-258-PB).

Blogs on Midkine

There are no specific blogs for Midkine, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Midkine
258-MD-010

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Midkine Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol MDK
Uniprot