Pleiotrophin/PTN was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human Pleiotrophin/PTN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-252-PB) at 3 ...read more
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Pleiotrophin/PTN Gly33-Asp168 Accession # P21246
Detects human Pleiotrophin/PTN in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross‑reactivity with recombinant mouse PTN is observed, and less than 1% cross‑reactivity with recombinant human Midkine is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Pleiotrophin/PTN Antibody [Unconjugated]
Heparin-binding brain mitogen
neurite growth-promoting factor 1
neurite growth-promoting factor1)
Osteoblast-specific factor 1
PTN was identified independently by several groups as a novel heparin-binding, developmentally regulated cytokine. Depending on the biological activities studied, this protein has variously been referred to as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding neurite promoting factor, heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), heparin-binding growth-associated molecule (HB-GAM), osteoblast-specific factor (OSF-1), and pleiotrophin. PTN is a highly conserved protein; the amino acid sequences of human, bovine, rat, and mouse PTN share >98% homology.
PTN is a member of a family of heparin-binding proteins that share sequence, structural, and functional similarity. Other members of this family include midkine (MK), and chicken retinoic acid-induced heparin-binding protein (RI-HB), an avian homologue of MK. The expression of all these cytokines is restricted and highly regulated during development.
PTN can be used as an attachment substrate to stimulate neurite outgrowth in mixed cultures of embryonic rat, mouse or chicken brain cells. Although both natural and recombinant human PTN have been reported to be mitogenic for fibroblasts, endothelial, and epithelial cells, the data are still highly controversial. PTN has been shown to transform NIH-3T3 and SW-13 cells, as evidenced by anchorage-independent growth and tumor formation in the nude mouse. These results suggest that, in spite of the conflicting reports of PTN’s growth-promoting activity in vitro, PTN may have a role in abnormal cell growth in vivo.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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