Pleiotrophin/PTN Antibody [Unconjugated]

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Pleiotrophin/PTN was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human Pleiotrophin/PTN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-252-PB) at 3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Pleiotrophin/PTN Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Pleiotrophin/PTN
Gly33-Asp168
Accession # P21246
Specificity
Detects human Pleiotrophin/PTN in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross‑reactivity with recombinant mouse PTN is observed, and less than 1% cross‑reactivity with recombinant human Midkine is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PTN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 3-15 ug/mL
Publications
Read Publications using
AF-252-PB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pleiotrophin/PTN Antibody [Unconjugated]

  • HARP
  • HBBM
  • HB-GAM
  • HBGF8
  • HBNF
  • HBNF1
  • HBNF-1
  • Heparin-binding brain mitogen
  • HNGF-8
  • NEGF1HBGF-8
  • neurite growth-promoting factor 1
  • neurite growth-promoting factor1)
  • OSF-1
  • Osteoblast-specific factor 1
  • Pleiotrophin
  • PTN

Background

PTN was identified independently by several groups as a novel heparin-binding, developmentally regulated cytokine. Depending on the biological activities studied, this protein has variously been referred to as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding neurite promoting factor, heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), heparin-binding growth-associated molecule (HB-GAM),
osteoblast-specific factor (OSF-1), and pleiotrophin. PTN is a highly conserved protein; the amino acid sequences of human, bovine, rat, and mouse PTN share >98% homology.

PTN is a member of a family of heparin-binding proteins that share sequence, structural, and functional similarity. Other members of this family include midkine (MK), and chicken retinoic acid-induced heparin-binding protein (RI-HB), an avian homologue of MK. The expression of all these cytokines is restricted and highly regulated during development.

PTN can be used as an attachment substrate to stimulate neurite outgrowth in mixed cultures of embryonic rat, mouse or chicken brain cells. Although both natural and recombinant human PTN have been reported to be mitogenic for fibroblasts, endothelial, and epithelial cells, the data are still highly controversial. PTN has been shown to transform NIH-3T3 and SW-13 cells, as evidenced by anchorage-independent growth and tumor formation in the nude mouse. These results suggest that, in spite of the conflicting reports of PTN’s growth-promoting activity in vitro, PTN may have a role in abnormal cell growth in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pleiotrophin/PTN Antibody (AF-252-PB)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Bioassay, ELISA Development (Capture), ICC, IHC, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development (Capture)
(1)
ICC
(2)
IHC
(1)
Neutralization
(5)
Western Blot
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF-252-PB Applications Species
HA Himburg, M Roos, T Fang, Y Zhang, CM Termini, L Schlussel, MM Kim, A Pang, J Kan, L Zhao, H Suh, JP Sasine, G Sapparapu, PM Bowers, G Schiller, JP Chute Chronic myelogenous leukemia stem cells require cell-autonomous pleiotrophin signaling J. Clin. Invest., 2019;0(0):. 2019 [PMID: 31613796] (Bioassay, Human) Bioassay Human
Wong J, Krueger K, Costa M, Aggarwal A, Du H, McLaughlin T, Feldman B A glucocorticoid- and diet-responsive pathway toggles adipocyte precursor cell activity in vivo. Sci Signal, 2016;9(451):ra103. 2016 [PMID: 27811141] (Neutralization, Human) Neutralization Human
Chen A, Donovan A, Ned-Sykes R, Andrews N Noncanonical role of transferrin receptor 1 is essential for intestinal homeostasis. Proc Natl Acad Sci U S A, 2015;112(37):11714-9. 2015 [PMID: 26324903] (IHC, Mouse) IHC Mouse
Fang Q, Mok P, Thomas A, Haddad D, Saini S, Clifford B, Kapasi N, Danforth O, Usui M, Ye W, Luu E, Sharma R, Bartel M, Pathmanabhan J, Ang A, Sievers R, Lee R, Springer M Pleiotrophin gene therapy for peripheral ischemia: evaluation of full-length and truncated gene variants. PLoS ONE, 2013;8(4):e61413. 2013 [PMID: 23630585] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Chen H, Campbell RA, Chang Y, Li M, Wang CS, Li J, Sanchez E, Share M, Steinberg J, Berenson A, Shalitin D, Zeng Z, Gui D, Perez-Pinera P, Berenson RJ, Said J, Bonavida B, Deuel TF, Berenson JR Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: a novel mechanism of tumor-induced vasculogenesis. Blood, 2009;113(9):1992-2002. 2009 [PMID: 19060246] (Neutralization, Human) Neutralization Human
Li J, Wei H, Chesley A, Moon C, Krawczyk M, Volkova M, Ziman B, Margulies KB, Talan M, Crow MT, Boheler KR The pro-angiogenic cytokine pleiotrophin potentiates cardiomyocyte apoptosis through inhibition of endogenous AKT/PKB activity. J. Biol. Chem., 2007;282(48):34984-93. 2007 [PMID: 17925408] (ICC, Human) ICC Human
Chen H, Gordon MS, Campbell RA, Li M, Wang CS, Lee HJ, Sanchez E, Manyak SJ, Gui D, Shalitin D, Said J, Chang Y, Deuel TF, Baritaki S, Bonavida B, Berenson JR Pleiotrophin is highly expressed by myeloma cells and promotes myeloma tumor growth. Blood, 2007;110(1):287-95. 2007 [PMID: 17369488] (Neutralization, Human) Neutralization Human
Marchionini DM, Lehrmann E, Chu Y, He B, Sortwell CE, Becker KG, Freed WJ, Kordower JH, Collier TJ Role of heparin binding growth factors in nigrostriatal dopamine system development and Parkinson's disease. Brain Res., 2007;1147(0):77-88. 2007 [PMID: 17368428] (Western Blot, Rat) Western Blot Rat
Li F, Shetty AK, Sugahara K Neuritogenic activity of chondroitin/dermatan sulfate hybrid chains of embryonic pig brain and their mimicry from shark liver. Involvement of the pleiotrophin and hepatocyte growth factor signaling pathways. J. Biol. Chem., 2007;282(5):2956-66. 2007 [PMID: 17145750] (Neutralization, Mouse) Neutralization Mouse
Gurok U, Steinhoff C, Lipkowitz B, Ropers HH, Scharff C, Nuber UA Gene expression changes in the course of neural progenitor cell differentiation. J. Neurosci., 2004;24(26):5982-6002. 2004 [PMID: 15229246] (ICC, Mouse) ICC Mouse
Show All 11 Publications.

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Secondary Antibodies

 

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Biotin BAF252

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Bioinformatics

Gene Symbol PTN
Uniprot