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HAF017). A specific band was detected for Midkine at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
HAF016). A specific band was detected for Midkine at approximately 15-17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
AF-258-PB) at 1.0 μg/ml. The Ponceau stained transfers of each blot are shown. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com)." class="big_lightbox" target="_blank">
Midkine (MK) is a 13 kDa heparin-binding polypeptide which enhances neurite outgrowth, neuronal cell survival and plasminogen activator activity. MK is structurally divided into two domains, and most of the biological activities are located on the C-terminal domain. Both domains consist of three antiparallel beta-strands, but the C-terminal domain has a long flexible hairpin loop where a heparin-binding consensus sequence is located (1). MK is a member of a highly conserved, developmentally regulated human gene family. The gene product exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Expression of MK is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable (2)
![Western Blot Midkine Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Midkine_AF258PB_Western_Blot_20090.jpg)
![Western Blot Midkine Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Midkine_AF7769_Western_Blot_12697.jpg)
![Immunocytochemistry Midkine Antibody (1011522) [Unconjugated]](http://images.novusbio.com/images/antibody/Midkine_MAB2582_Immunocytochemistry__Immunofluorescence_23704.jpg)
![N/A Midkine [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Midkine_DY258_ELISA_1850.jpg)
![Immunohistochemistry Midkine Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Midkine_AF-258-PB_Immunohistochemistry_10187.jpg)
![Immunohistochemistry Midkine Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Midkine_AF-258-PB_Immunohistochemistry_9768.jpg)
![Simple Western Midkine Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Midkine_AF258PB_Simple_Western_20091.jpg)
![Knockout Validated Midkine Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-258-pb_human-midkine-affinity-purified-polyclonal-ab-knockout-validated-89202294520.jpg)
![Immunoprecipitation Midkine Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-258-pb_human-midkine-affinity-purified-polyclonal-ab-immunoprecipitation-2211202214158.jpg)
![Immunohistochemistry Midkine Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Midkine_AF7769_Immunohistochemistry_12696.jpg)
![Simple Western Midkine Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Midkine_AF7769_Simple_Western_20087.jpg)
![Western Blot Midkine Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af7769_mouse-midkine-affinity-purified-pab-western-blot-121220257465817.jpg)
![Immunohistochemistry Midkine Antibody (1011522) [Unconjugated]](http://images.novusbio.com/images/antibody/Midkine_MAB2582_Immunohistochemistry_23705.jpg)
![Immunoprecipitation Midkine Antibody (1011522) [Unconjugated]](http://images.novusbio.com/images/mab2582_human-midkine-mab-clone-1011522-immunoprecipitation-22112022135356.jpg)
