MD-2 Antibody

Images

 
Western Blot: MD-2 Antibody [NBP1-77201] - Analysis of MD2 in mouse spleen cell lysate at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: MD-2 Antibody [NBP1-77201] - Staining of Rat Spleen cells
Immunohistochemistry: MD-2 Antibody [NBP1-77201] - Analysis of MD-2 in human spleen tissue with MD-2 antibody at 2.5 ug/ml.
Immunohistochemistry-Paraffin: MD-2 Antibody [NBP1-77201] - Rat spleen cells at 2 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MD-2 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 13 amino acids near the center of human MD-2. The immunogen is located within the last 50 amino acids of MD-2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LY96
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence 10 ug/ml
Application Notes
MD-2 antibody can be used for detection of MD-2 by Western blot at 0.5 to 2 mg/ml.
Control Peptide
MD-2 Peptide (NBP1-77201PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MD-2 Antibody

  • ESOP1
  • ESOP-1
  • LY96
  • ly-96
  • lymphocyte antigen 96
  • MD2
  • MD-2
  • myeloid differentiation protein-2
  • Protein MD-2

Background

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LY96
Entrez
Uniprot