MD-2 Antibody - BSA Free

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Rat Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MD-2 recombinant protein per lane. Antibodies: MD-2, NBP1-77201 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL and Lane 3: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Daudi Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Mouse Spleen Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: MD-2, NBP1-77201 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Mouse Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Human Spleen Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling MD-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Mouse Spleen Tissue LysateLoading: 15 ug of lysates per lane.Antibodies: MD-2, (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MD-2 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Rat Tissue Lysates.Loading: 15 ug of lysates per lane.Antibodies: MD-2, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Rat Spleen Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed rat spleen tissue labeling MD-2 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen using anti-MD-2 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Rat Spleen Tissue Lysate.Loading: 15 ug of lysates per lane.Antibodies: MD-2, (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MD-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MD-2 Antibody - BSA Free (NBP1-77201) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-MD-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 13 amino acids near the center of human MD-2. The immunogen is located within the last 50 amino acids of MD-2. Amino Acid Sequence: CSFKGIKFSKGKYK
• Specificity: Human MD-2 has 2 isoforms, including isoform 1 (160aa, 18kD) and isoform 2 (130aa, 15kD). Mouse MD-2 has one isoform (160aa, 18kD) and Rat MD-2 also has one isoform (160aa, 18kD). NBP1-77201 can detect human, mouse and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LY96
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/mL
  • Immunohistochemistry 2-2.5 ug/mL
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 0.5-1 ug/mL
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  MD-2 Peptide (NBP1-77201PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for MD-2 Antibody - BSA Free

• ESOP1
• ESOP-1
• LY96
• ly-96
• lymphocyte antigen 96
• MD2
• MD-2
• myeloid differentiation protein-2
• Protein MD-2

Background

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MD-2 Antibody (NBP1-77201)(1)

Publication using NBP1-77201

• Koyama D, Maruoka S et al. Myeloid differentiation-2 is a potential biomarker for the amplification process of allergic airway sensitization in mice. Allergol Int 2015-01-09 [PMID: 26344079] (IF/IHC, ELISA, Mouse)

Bioinformatics

• Gene Symbol: LY96
• Uniprot:
  • NP_056179(Human)