MCM4 Antibody

Images

 
Western Blot: MCM4 Antibody [NB100-290] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.
Immunohistochemistry: MCM4 Antibody [NB100-290] - Sample: FFPE section of human testicular seminoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-MCM4 used at a dilution of ...read more
Proximity Ligation Assay: MCM4 Antibody [NB100-290] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RPA2 (NBP2-22232) and rabbit anti-human MCM4 (NB100-290). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: MCM4 Antibody [NB100-290] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human USP7 (NBP2-22295) and rabbit anti-human MCM4 (NB100-290). Image merged from DAPI (2ms), FITC/Green ...read more

Product Details

Summary
Reactivity Hu, Mu, XpSpecies Glossary
Applications WB, IHC, PLA, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MCM4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 750 and 800 of human Minichromosome Maintenance 4 using the numbering given in entry NP_005905.2 (GeneID 4173).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCM4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Proximity Ligation Assay 1:2000-1:40000
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publication using NB100-290.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MCM4 Antibody

  • CDC21 homolog
  • CDC21
  • CDC54
  • DNA replication licensing factor MCM4
  • EC 3.6.4.12
  • hCdc21
  • homolog of S. pombe cell devision cycle 21
  • MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)
  • MCM4 minichromosome maintenance deficient 4
  • MGC33310
  • minichromosome maintenance complex component 4
  • minichromosome maintenance deficient 4
  • P1-Cdc21

Background

The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM4 is part of the core MCM complex that includes MCM4, MCM6, and MCM7. The core complex possesses DNA helicase activity. MCM4 is phosphorylated by CDC2 kinase resulting in a reduction of helicase activity and chromatin binding. Alternate names for MCM4 include DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21, CDC54, hCdc21, and MGC33310.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCM4 Antibody (NB100-290)(1)

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MCM4 Antibody (NB100-290)

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Bioinformatics

Gene Symbol MCM4
Entrez
Uniprot