CDT1 Antibody

Images

 
Western Blot: CDT1 Antibody [NB100-2567] - Human B cells. KSHV infection protects CDT1 from hypoxia mediated degradation. Mock or KSHV infected cells were grown under normoxic or hypoxic condition followed by measuring ...read more
Immunohistochemistry-Paraffin: CDT1 Antibody [NB100-2567] - Human islet cell carcinoma. Antibody used at a dilution of 1:1000 (1ug/mL).
Western Blot: CDT1 Antibody [NB100-2567] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti- CDT1 antibody (NB100-2567) used ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CDT1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 546) of human Chromatin Licensing and DNA Replication Factor 1 using the numbering given in entry NP_112190.1 (GeneID 81620).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:2500
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Theoretical MW
60.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
CDT1 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 3.3
using
NB100-2567 in the following applications:

Publications
Read Publications using
NB100-2567 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7 - 8)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDT1 Antibody

  • chromatin licensing and DNA replication factor 1
  • DNA replication factor Cdt1
  • Double parked homolog
  • Double parked, Drosophila, homolog of
  • DUPRIS2

Background

Geminin is a protein that inhibits DNA replication by preventing the loading of MCM complex on chromatin. Human CDT1 is a 65kDa protein that contains 546 amino acids and coimmunoprecipitates with Geminin. Inhibition of DNA replication by Geminin in cell-free cDNA replication extracts can be reversed by the addition of excess CDT1. In the normal cell cycle, CDT1 is present only in G1 and S phases, whereas Geminin is present in S and G2 phases. Wohlschlegel et al. (2000) concluded that their results suggest that Geminin inhibits inappropriate origin firing by targeting CDT1. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animals cells produce the protein, which stimulates the animals immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDT1 Antibody (NB100-2567)(5)

Reviews for CDT1 Antibody (NB100-2567) (3) 3.33

Average Rating: 3.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB100-2567:
Filter by Applications
WB
(2)
ICC
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot CDT1 NB100-2567
Enlarge
4
reviewed by:
Rajnish Singh
WB Human 10/16/2019
View

Summary

ApplicationWestern Blot
Sample TestedB cells
SpeciesHuman
LotA1
Immunocytochemistry CDT1 NB100-2567
Enlarge
1
reviewed by:
Anonymous
ICC Human 01/17/2018
View

Summary

ApplicationImmunocytochemistry
Sample Tested hela cell
SpeciesHuman
LotA1

Comments

CommentsFixed in 4% PFA for 10 min, permeablized in.2% Triton X-100 in PBS for 15 min, blocked for 1 hr in 1% BSA + 10% FBS in PBS with.1% Triton X-100. Incubated in antibody at 1:100 dilution in blocking buffer, and detected with anti-rabbit secondary antibody.
Western Blot CDT1 NB100-2567
Enlarge
5
reviewed by:
Maimon Hubbi
WB Human 02/18/2011
View

Summary

ApplicationWestern Blot
Sample TestedHCT116 Whole Cell Lysate, Sample Amount: 50 ug
SpeciesHuman
CommentsClear detection of endogenous Cdt1 from HCT116 cell lysates. Single band at expected molecular weight.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: overnight, Incubation Temp: 4 C

Comments

CommentsClear detection of endogenous Cdt1 from HCT116 cell lysates. Single band at expected molecular weight.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CDT1 Antibody (NB100-2567) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2567AF350
Alexa Fluor 405 NB100-2567AF405
Alexa Fluor 488 NB100-2567AF488
Alexa Fluor 532 NB100-2567AF532
Alexa Fluor 594 NB100-2567AF594
Alexa Fluor 647 NB100-2567AF647
Alexa Fluor 700 NB100-2567AF700
Alexa Fluor 750 NB100-2567AF750
Biotin NB100-2567B
DyLight 350 NB100-2567UV
DyLight 405 NB100-2567V
DyLight 488 NB100-2567G
DyLight 550 NB100-2567R
DyLight 594 NB100-2567DL594
DyLight 650 NB100-2567C
DyLight 680 NB100-2567FR
DyLight 755 NB100-2567IR
FITC NB100-2567F
HRP NB100-2567H
Janelia Fluor 549 NB100-2567JF549
Janelia Fluor 646 NB100-2567JF646

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Recent Reviews

3.3
5
1
4
1
3
0
2
0
1
1

Rajnish Singh
10/16/2019
Application: WB
Species: Human

Anonymous
01/17/2018
Application: ICC
Species: Human

Maimon Hubbi
02/18/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol CDT1
Entrez
Uniprot