DBF4 Antibody (6G9)

Western Blot: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.Lane 1: DBF4 transfected lysate(76.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of monoclonal antibody to DBF4 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: DBF4 Antibody (6G9) [H00010926-M01] - Immunoperoxidase of monoclonal antibody to DBF4 ( Cat # H00010926-M01 ) on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]

Sandwich ELISA: DBF4 Antibody (6G9) [H00010926-M01] - Detection limit for recombinant GST tagged DBF4 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 6G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DBF4 Antibody (6G9) Summary

• Immunogen: DBF4 (NP_006707, 2 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQT
• Specificity: DBF4 - DBF4 homolog (S. cerevisiae)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DBF4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DBF4 Antibody (6G9)

• ASKchif
• DBF4 homolog (S. cerevisiae)
• DBF4ACHIF
• DBF4-type zinc finger-containing protein 1
• ZDBF1DBF-type containing 1

Background

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DBF4 Antibody (H00010926-M01)(1)

Publication using H00010926-M01

• Hughes S, Jenkins V, Dar MJ et al. Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. J Biol Chem 2010-01-01 [PMID: 19864417]

Bioinformatics

• Gene Symbol: DBF4
• Entrez:
  • 10926(Human)
• OMIM:
  • 604281(Human)
• Uniprot:
  • NP_006707(Human)