Cdc6 Antibody

Western Blot: Cdc6 Antibody [NBP2-15837] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CDC6 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Cdc6 Antibody [NBP2-15837] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Western Blot: Cdc6 Antibody [NBP2-15837] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug Raw264. 7 whole cell lysate/ extract 7.5% SDS Page Gel, antibody diluted 1:1000

Western Blot: Cdc6 Antibody [NBP2-15837] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract7. 5 % SDS-PAGECdc6 antibody dilution: 1:1000.

Western Blot: Cdc6 Antibody [NBP2-15837] - Sample (30 ug of whole cell lysate) A: JurKat 7. 5% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cdc6 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cdc6. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:10 - 1:500
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rat (80%), Bovine (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0) and 20% glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Cdc6 Antibody

• CDC18LHsCDC18
• Cdc18-related protein
• CDC6 (cell division cycle 6, S. cerevisiae) homolog
• CDC6 cell division cycle 6 homolog (S. cerevisiae)
• CDC6 cell division cycle 6 homolog
• CDC6-related protein
• cell division control protein 6 homolog
• cell division cycle 6 homolog (S. cerevisiae)
• cell division cycle 6 protein
• HsCdc18
• HsCDC6
• p62(cdc6)

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC6
• Uniprot:
  • Q99741(Mouse)