MCM2 Antibody (6A8) - Azide and BSA Free

Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of monoclonal antibody to MCM2 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - MCM2 monoclonal antibody (M01), clone 6A8 Analysis of MCM2 expression in Hela S3 NE.

Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 MaxPab rabbit polyclonal antibody.

ELISA: MCM2 Antibody (6A8) [H00004171-M01] - Detection limit for recombinant GST tagged MCM2 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 6A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MCM2 Antibody (6A8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MCM2 (AAH07670, 805 a.a. ~ 904 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF
• Specificity: MCM2 - MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCM2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27976684).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MCM2 Antibody (6A8) - Azide and BSA Free

• BM28
• BM28MGC10606
• CCNL 1
• CCNL1
• cdc19
• CDCL1
• CDCL1mitotin
• D3S3194
• EC 3.6.4.12
• KIAA0030MITOTIN
• MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
• MCM2
• minichromosome maintenance complex component 2
• minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
• minichromosome maintenance deficient 2 (mitotin)
• Mitotin

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCM2 Antibody (H00004171-M01)(6)

Publications using H00004171-M01

• Polasek-Sedlackova H, Miller TCR, Krejci J et al. Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes Nature communications 2022-10-14 [PMID: 36241664] (ICC/IF, Human)
• Zonderland G, Vanzo R, Amitash S et al. The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition Molecular cell 2022-08-23 [PMID: 36049481] (ICC/IF)
• Changsheng Zhang, Shengyang Du, Lei Cao Retracted Article: Long non-coding RNA KCNQ1OT1 promotes osteosarcoma progression by increasing beta -catenin activity RSC Advances 2018-11-08 [PMID: 35558611] (Western Blot, Human)
• Ochs, F, Karemore, G Et al. Stabilization of chromatin topology safeguards genome integrity. Nature 2019-10-01 [PMID: 31645724] (Western Blot, Human)
• S Pedersen R, Karemore G, Gudjonsson T et al. Profiling DNA damage response following mitotic perturbations. Nat Commun. 2016-12-15 [PMID: 27976684] (WB, Mouse)
• Ivanov SV, Goparaju CM, Lopez P et al. Pro-tumorigenic effects of miR-31 loss in mesothelioma. J Biol Chem 2010-07-23 [PMID: 20463022] (WB, Human)

Bioinformatics

• Gene Symbol: MCM2
• Entrez:
  • 4171(Human)
• OMIM:
  • 116945(Human)
• Uniprot:
  • AAH07670(Human)