Biological Strategies: Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. ...read more
Immunohistochemistry: MCM5 Antibody [NB100-79778] - Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 used at a dilution of ...read more
Western Blot: MCM5 Antibody [NB100-79778] - Whole cell lysate (3.8, 7.5 or 15 ug for lanes 1, 2 and 3, respectively) from HeLa cells. Antibody used at 0.2 ug/ml.
MCM5 (Mini Chromosome Maintainance protein-5) is involved in the control of DNA replication. MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increase as the cell enters G1/S phase of the cell cycle. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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