MCM5 Antibody

Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: MCM5 Antibody [NB100-79778] - Human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

MCM5 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Minichromosome Maintenance 5 using the numbering given in entry NP_006730.2 (GeneID 4174).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MCM5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-4 ug/mg lysate
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is efficient at pulling down the MCM2-7 complex. Use in ICC/IF reported in scientific literature (PMID: 25071185). Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Control:

  MCM5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MCM5 Antibody

• CDC46 homolog
• CDC46DNA replication licensing factor MCM5
• EC 3.6.4.12
• MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S.cerevisiae)
• MCM5 minichromosome maintenance deficient 5, cell division cycle 46
• MGC5315
• minichromosome maintenance complex component 5
• minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46)
• minichromosome maintenance deficient 5 (cell division cycle 46)
• P1-CDC46

Background

MCM5 (Mini Chromosome Maintainance protein-5) is involved in the control of DNA replication. MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increase as the cell enters G1/S phase of the cell cycle. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCM5 Antibody (NB100-79778)(6)

Publications using NB100-79778

• Hubbi ME, Gilkes DM, Hu H et al. Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1alpha to promote cell-cycle progression. Proc Natl Acad Sci U S A. 2014-07-28 [PMID: 25071185] (ICC/IF, Human)
• Panneerselvam J, Pickering A, Han B et al. Basal level of FANCD2 monoubiquitination is required for the maintenance of a sufficient number of licensed-replication origins to fire at a normal rate. Oncotarget 2014-07-15 [PMID: 24658369] (WB, Human)
  
  Details:
  MCM6 antibody used for WB on several experiments involving CRL-1790 and HEK293T cells.
• Hubbi ME, Kshitiz, Gilkes DMet al. A nontranscriptional role for HIF-1alpha as a direct inhibitor of DNA replication. Sci Signal 2013-02-12 [PMID: 23405012]
• Hubbi ME, Luo W, Baek JH et al. MCM proteins are negative regulators of hypoxia-inducible factor 1 Mol Cell 2011-06-10 [PMID: 21658608] (WB, Human)
• Yan S, Michael WM. TopBP1 and DNA polymerase-alpha directly recruit the 9-1-1 complex to stalled DNA replication forks. J Cell Biol 2009-03-01 [PMID: 19289795]
• Yan S, Lindsay HD, Michael WM et al. Direct requirement for Xmus101 in ATR-mediated phosphorylation of Claspin bound Chk1 during checkpoint signaling. J Cell Biol 2006-04-01 [PMID: 16618813]

Bioinformatics

• Gene Symbol: MCM5
• Entrez:
  • 4174(Human)
• Uniprot:
  • NP_006730.2(Human)