MCM5 Antibody

Images

 
Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry: MCM5 Antibody [NB100-79778] - Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 used at a dilution of ...read more
Western Blot: MCM5 Antibody [NB100-79778] - Whole cell lysate (3.8, 7.5 or 15 ug for lanes 1, 2 and 3, respectively) from HeLa cells. Antibody used at 0.2 ug/ml.
Proximity Ligation Assay: MCM5 Antibody [NB100-79778] - Secondary-conjugate Duolink II PLA in Hela Cells. Goat anti-human RPA2 (NBP2-22232) and rabbit anti-human MCM5 (NB100-79778). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: MCM5 Antibody [NB100-79778] - Secondary-conjugate Duolink in Situ PLA in Hela cells. goat anti-human USP7 (NBP2-22295) and rabbit anti-human MCM5 (NB100-79778). Image merged from DAPI (2ms), ...read more

Product Details

Summary
Reactivity Hu, XpSpecies Glossary
Applications WB, IHC, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

MCM5 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Minichromosome Maintenance 5 using the numbering given in entry NP_006730.2 (GeneID 4174).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCM5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunohistochemistry
  • Proximity Ligation Assay
Application Notes
Western blot. This antibody is efficient at pulling down the MCM2-7 complex
Positive Control
MCM5 Lysate (NBL1-12954)
Publications
Read Publications using
NB100-79778 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MCM5 Antibody

  • CDC46 homolog
  • CDC46DNA replication licensing factor MCM5
  • EC 3.6.4.12
  • MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S.cerevisiae)
  • MCM5 minichromosome maintenance deficient 5, cell division cycle 46
  • MGC5315
  • minichromosome maintenance complex component 5
  • minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46)
  • minichromosome maintenance deficient 5 (cell division cycle 46)
  • P1-CDC46

Background

MCM5 (Mini Chromosome Maintainance protein-5) is involved in the control of DNA replication. MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increase as the cell enters G1/S phase of the cell cycle. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCM5 Antibody (NB100-79778)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(2)
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(3)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MCM5 Antibody (NB100-79778) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MCM5
Entrez
Uniprot