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HAF018). A specific band was detected for MCM4 at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human MCM4 Monoclonal Antibody (Catalog # MAB11718) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the Jurkat human acute T cell leukemia cell line." class="big_lightbox" target="_blank">
VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
MCM4 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
![Western Blot MCM4 Antibody (1099134) [Unconjugated]](http://images.novusbio.com/images/antibody/mab11718_human-mcm4-mab-clone-1099134-western-blot-297202515853..jpg)
![Simple Western MCM4 Antibody (1099134) [Unconjugated]](http://images.novusbio.com/images/antibody/mab11718_human-mcm4-mab-clone-1099134-simple-western-2972025151342..jpg)
![Immunohistochemistry MCM4 Antibody (1099134) [Unconjugated]](http://images.novusbio.com/images/antibody/mab11718_human-mcm4-mab-clone-1099134-immunohistochemistry-172025103216..jpg)
![Immunohistochemistry MCM4 Antibody (1099134) [Unconjugated]](http://images.novusbio.com/images/antibody/mab11718_human-mcm4-mab-clone-1099134-immunohistochemistry-17202510338..jpg)
![Immunohistochemistry MCM4 Antibody (1099134) [Unconjugated]](http://images.novusbio.com/images/antibody/mab11718_human-mcm4-mab-clone-1099134-immunohistochemistry-17202510343..jpg)
![Immunocytochemistry MCM4 Antibody (1099134) [Unconjugated]](http://images.novusbio.com/images/antibody/mab11718_human-mcm4-mab-clone-1099134-immunocytochemistry-172025103746..jpg)
![Immunocytochemistry MCM4 Antibody (1099134) [Unconjugated]](http://images.novusbio.com/images/antibody/mab11718_human-mcm4-mab-clone-1099134-immunocytochemistry-172025103920..jpg)
