MCAM/CD146 Antibody (404722) [Unconjugated]

PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat MCAM/CD146 Monoclonal Antibody (Catalog # MAB3250, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: WB, Flow, CyTOF-ready
• Clone: 404722
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MCAM/CD146 Antibody (404722) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
  Arg19-Lys560
  Accession # Q9EPF2
• Specificity: Detects rat MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) MCAM, rhBCAM, or rhALCAM is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCAM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCAM/CD146 Antibody (404722) [Unconjugated]

• CD146 antigen
• CD146
• cell surface glycoprotein MUC18
• Cell surface glycoprotein P1H12
• L-Gicerin
• MCAM
• melanoma adhesion molecule
• melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
• Melanoma-associated antigen A32
• Melanoma-associated antigen MUC18
• MUC18
• MUC18Gicerin

Background

Melanomia Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is a putative Ig-superfamily adhesion molecule that is expressed on endothelial cells and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCAM/CD146 Antibody (MAB3250)(2)

Publications using MAB3250

• ZCT Zaw, N Kawashima, T Kaneko, T Okiji Angiogenesis during coronal pulp regeneration using rat dental pulp cells: Neovascularization in rat molars in�vivo and proangiogenic dental pulp cell-endothelial cell interactions in�vitro Journal of dental sciences, 2022-02-05;17(3):1160-1168. 2022-02-05 [PMID: 35784152] (IHC, Rat)
• TQ Kieu, K Tazawa, N Kawashima, S Noda, M Fujii, K Nara, K Hashimoto, P Han, T Okiji Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro Scientific Reports, 2022-03-25;12(1):5176. 2022-03-25 [PMID: 35338195] (ICC, IHC, Mouse)

Bioinformatics

• Gene Symbol: MCAM
• Uniprot:
  • Q9EPF2()