Mannose Phosphate Isomerase Antibody

Images

 
Western Blot: Mannose Phosphate Isomerase Antibody [NBP1-31460] - Wild-type (WT) and MPI knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MPI antibody. The ...read more
Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody [NBP1-31460] - Paraffin-embedded Gastric CA N87 xenograft, using antibody at 1:100 dilution.
Western Blot: Mannose Phosphate Isomerase Antibody [NBP1-31460] - Sample(30ug whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 10% SDS PAGE, antibody diluted at 1:1000.
Western Blot: Mannose Phosphate Isomerase Antibody [NBP1-31460] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE MPI antibody dilution: 1:1000

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Order Details

Mannose Phosphate Isomerase Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MPI. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MPI
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (84%), Chimpanzee (99%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Mannose Phosphate Isomerase Antibody

  • CDG1B
  • EC 5.3.1
  • EC 5.3.1.8
  • FLJ39201
  • mannose phosphate isomerase
  • mannose-6- phosphate isomerase
  • mannose-6-phosphate isomerase
  • Phosphohexomutase
  • phosphomannose isomerase 1
  • Phosphomannose isomerase
  • PMI
  • PMI1phosphohexomutase

Background

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MPI