M-CSFR/CD115 Antibody [Biotin]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse M-CSF R/CD115 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF3818, filled histogram) or control antibody (Catalog # BAF020, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

• Reactivity: Mu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

M-CSFR/CD115 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse M-CSF R/CD115
  Ala20-Ser511
  Accession # P09581
• Specificity: Detects mouse M-CSF R/CD115 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human
  M-CSF R/CD115 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CSF1R
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-CSFR/CD115 Antibody [Biotin]

• CD115 antigen
• CD115
• c-fms
• colony stimulating factor 1 receptor
• CSF1R
• CSF-1-R
• CSFR
• EC 2.7.10.1
• FMS proto-oncogene
• FMSFIM2
• macrophage colony stimulating factor I receptor
• macrophage colony-stimulating factor 1 receptor
• McDonough feline sarcoma viral (v-fms) oncogene homolog
• M-CSF R
• MCSFR
• M-CSFR
• Proto-oncogene c-Fms

Background

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Mouse M-CSF receptor cDNA encodes a 977 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 492 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 441 aa cytoplasmic domain. The mouse M-CSF R ECD shares >99% aa identity with rat and 60-63% aa identity with corresponding sequences in human, canine, feline and bovine M-CSF R. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation, and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts, and DC (3, 4). M-CSF R and integrin alpha _v beta ₃ share signaling pathways during osteoclastogenesis, and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
7. Dai, X. et al. (2002) Blood 99:111.
8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
9. Li, M. et al. (2004) J. Neurochem. 91:623.
10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for M-CSFR/CD115 Antibody (BAF3818)(2)

Publications using BAF3818

• H Lundgren, K Martinsson, K Cederbrant, J Jirholt, D Mucs, K Madeyski-B, S Havarinasa, P Hultman HLA-DR7 and HLA-DQ2: Transgenic mouse strains tested as a model system for ximelagatran hepatotoxicity PLoS ONE, 2017-09-21;12(9):e0184744. 2017-09-21 [PMID: 28934241] (ELISA Development (Detection), Mouse)
• Arai A, Mizoguchi T, Harada S, Kobayashi Y, Nakamichi Y, Yasuda H, Penninger J, Yamada K, Udagawa N, Takahashi N Fos plays an essential role in the upregulation of RANK expression in osteoclast precursors within the bone microenvironment. J Cell Sci, 2012-03-27;125(0):2910-7. 2012-03-27 [PMID: 22454522] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: CSF1R
• Uniprot:
  • P09581()