M-CSFR/CD115 Antibody (AFS98) [Biotin]

Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [Biotin] [NBP1-43592] - Staining of thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram). Cells in the large scatter population were used for analysis.

Product Discontinued

M-CSFR/CD115 Antibody (AFS98) [Biotin] Summary

• Immunogen: The immunogen for this antibody was CD115.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CSF1R
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Blocking/Neutralizing reported in scientific literature (PMID: 24056963)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for M-CSFR/CD115 Antibody (AFS98) [Biotin]

• CD115 antigen
• CD115
• c-fms
• colony stimulating factor 1 receptor
• CSF1R
• CSF-1-R
• CSFR
• EC 2.7.10.1
• FMS proto-oncogene
• FMSFIM2
• macrophage colony stimulating factor I receptor
• macrophage colony-stimulating factor 1 receptor
• McDonough feline sarcoma viral (v-fms) oncogene homolog
• M-CSF R
• MCSFR
• M-CSFR
• Proto-oncogene c-Fms

Background

The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF1R
• Entrez:
  • 1436(Human)
• Uniprot:
  • P07333(Human)