M-CSFR/CD115 Antibody (AFS98)

Images

 
Immunohistochemistry-Frozen: M-CSFR/CD115 Antibody (AFS98) [NBP1-43363] - Mouse brain section. Antibody at 1:200. IHC-Fr image submitted by a verified customer review.
Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Analysis using the Biotin conjugate of NBP1-43363. Staining of thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD115 (c-fms) PE. Appropriate ...read more
Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Staining of thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram). Cells ...read more
Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Staining of thioglycolate-induced peritoneal exudate cells (PECs) with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (NBP1-43321) (open histogram) or ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, B/N
Clone
AFS98
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

M-CSFR/CD115 Antibody (AFS98) Summary

Immunogen
The immunogen for this antibody was CD115.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CSF1R
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry < 1 ug/10^6 cells
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Block/Neutralize
Application Notes
The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Blocking/Neutralizing reported in scientific literature (PMID: 24056963)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-43363 in the following applications:

Publications
Read Publications using
NBP1-43363 in the following applications:

  • B/N
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for M-CSFR/CD115 Antibody (AFS98)

  • CD115 antigen
  • CD115
  • c-fms
  • colony stimulating factor 1 receptor
  • CSF1R
  • CSF-1-R
  • CSFR
  • EC 2.7.10.1
  • FMS proto-oncogene
  • FMSFIM2
  • macrophage colony stimulating factor I receptor
  • macrophage colony-stimulating factor 1 receptor
  • McDonough feline sarcoma viral (v-fms) oncogene homolog
  • M-CSF R
  • MCSFR
  • M-CSFR
  • Proto-oncogene c-Fms

Background

The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for M-CSFR/CD115 Antibody (NBP1-43363)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: B/N.


Filter By Application
B/N
(1)
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Filter By Species
Mouse
(1)
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Review for M-CSFR/CD115 Antibody (NBP1-43363) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-43363:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen M-CSFR/CD115 NBP1-43363
Enlarge
4
reviewed by:
Abhijit Nirwane
IHC-Fr Mouse 09/18/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Mouse brain
SpeciesMouse
Lot4282783

Comments

CommentsDilution 1:200

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for M-CSFR/CD115 Antibody (NBP1-43363) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-43363AF350
Alexa Fluor 405 NBP1-43363AF405
Alexa Fluor 488 NBP1-43363AF488
Alexa Fluor 532 NBP1-43363AF532
Alexa Fluor 594 NBP1-43363AF594
Alexa Fluor 647 NBP1-43363AF647
Alexa Fluor 700 NBP1-43363AF700
Alexa Fluor 750 NBP1-43363AF750
Allophycocyanin NBP1-43363APC
Allophycocyanin/Cy7 NBP1-43363APCCY7
Biotin NBP1-43592
Biotin NBP1-43592
Biotin NBP1-43592
DyLight 350 NBP1-43363UV
DyLight 405 NBP1-43363V
DyLight 488 NBP1-43363G
DyLight 550 NBP1-43363R
DyLight 594 NBP1-43363DL594
DyLight 650 NBP1-43363C
DyLight 680 NBP1-43363FR
DyLight 755 NBP1-43363IR
Janelia Fluor 549 NBP1-43363JF549
Janelia Fluor 646 NBP1-43363JF646
PE NBP1-43363PE
PE/Atto594 NBP1-43363PEATT594
PE/Cy5.5 NBP1-43363PECY55
PE/Cy7 NBP1-43363PECY7
PerCP NBP1-43363PCP

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Bioinformatics Tool for M-CSFR/CD115 Antibody (NBP1-43363)

Discover related pathways, diseases and genes to M-CSFR/CD115 Antibody (NBP1-43363). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Abhijit Nirwane
09/18/2019
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol CSF1R
Entrez
Uniprot