M-CSFR/CD115 Antibody (AFS98) - BSA Free

Images

 
Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (AFS98) [NBP1-43363] - Mouse tumor tissue stained with M-CSFR/CD115 Antibody (AFS98). Tissue sections were deparaffinized and incubated with the antibody O/N at 4C. ...read more
Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Analysis using the Biotin conjugate of NBP1-43363. Staining of thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD115 (c-fms) PE. Appropriate ...read more
Immunohistochemistry-Frozen: M-CSFR/CD115 Antibody (AFS98) [NBP1-43363] - Mouse brain section. Antibody at 1:200. IHC-Fr image submitted by a verified customer review.
Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Staining of thioglycolate-induced peritoneal exudate cells with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram). Cells ...read more
Flow Cytometry: M-CSF R/CD115 Antibody (AFS98) [NBP1-43363] - Staining of thioglycolate-induced peritoneal exudate cells (PECs) with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (NBP1-43321) (open histogram) or ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P, B/N
Clone
AFS98
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

M-CSFR/CD115 Antibody (AFS98) - BSA Free Summary

Immunogen
The immunogen for this antibody was CD115.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CSF1R
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Flow Cytometry < 1 ug/10^6 cells
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100 - 1:2000
Application Notes
The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Blocking/Neutralizing reported in scientific literature (PMID: 24056963). This M-CSFR/CD115 Antibody (AFS98) is validated for IHC-P from a verified customer review.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-43363 in the following applications:

Publications
Read Publications using
NBP1-43363 in the following applications:

  • B/N
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for M-CSFR/CD115 Antibody (AFS98) - BSA Free

  • CD115 antigen
  • CD115
  • c-fms
  • colony stimulating factor 1 receptor
  • CSF1R
  • CSF-1-R
  • CSFR
  • EC 2.7.10.1
  • FMS proto-oncogene
  • FMSFIM2
  • macrophage colony stimulating factor I receptor
  • macrophage colony-stimulating factor 1 receptor
  • McDonough feline sarcoma viral (v-fms) oncogene homolog
  • M-CSF R
  • MCSFR
  • M-CSFR
  • Proto-oncogene c-Fms

Background

The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for M-CSFR/CD115 Antibody (NBP1-43363)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: B/N.


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B/N
(1)
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Mouse
(1)
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Reviews for M-CSFR/CD115 Antibody (NBP1-43363) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP1-43363:
Filter by Applications
IF
(1)
Immunohistochemistry-Frozen
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
IF M-CSFR/CD115 NBP1-43363
Enlarge
5
reviewed by:
Michael Nowak
IF Mouse 11/09/2021
View

Summary

ApplicationIF
Sample TestedTumor tissue
SpeciesMouse
LotAFS98

Comments

CommentsTissue sections were deparaffinized. Incubated O/N in 4 C with the 1ry antibody.
Immunohistochemistry-Frozen M-CSFR/CD115 NBP1-43363
Enlarge
4
reviewed by:
Verified Customer
Immunohistochemistry-Frozen Mouse 09/18/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse brain
SpeciesMouse
Lot4282783

Comments

CommentsDilution 1:200

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FAQs for M-CSFR/CD115 Antibody (NBP1-43363) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for M-CSFR/CD115 Antibody (NBP1-43363)

Discover related pathways, diseases and genes to M-CSFR/CD115 Antibody (NBP1-43363). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Michael Nowak
11/09/2021
Application: IF
Species: Mouse

Verified Customer
09/18/2019
Application: Immunohistochemistry-Frozen
Species: Mouse

Bioinformatics

Gene Symbol CSF1R
Entrez
Uniprot